Integration of root architecture, root nitrogen metabolism, and photosynthesis of ‘Hanfu’ apple trees under the cross-talk between glucose and IAA
Integration of root architecture, root nitrogen metabolism, and photosynthesis of ‘Hanfu’ apple trees under the cross-talk between glucose and IAA
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DOI：
10.1016/j.hpj.2022.12.009
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发表时间：
2022-12
影响因子：
5.7
通讯作者：
Bian Qi;Xin Zhang;Z. Mao;S. Qin;Deguo Lv
中科院分区：
文献类型：
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作者：
Bian Qi;Xin Zhang;Z. Mao;S. Qin;Deguo Lv
糖和生长素几乎对植物生命周期的所有阶段都有重要影响，它们是植物的基础，并调节着类似的过程。然而，葡萄糖与吲哚-3-乙酸（IAA）互扰对苹果树生长发育的影响尚不清楚。为了研究葡萄糖和IAA在汉赋（Malus domestica）根系构型、根系氮代谢和光合能力中的潜在作用，建立了5个处理：葡萄糖、IAA和生长素极性运输抑制剂（2,3,5 -三碘苯甲酸，TIBA）单施、葡萄糖与TIBA和葡萄糖与IAA联合施用。葡萄糖与IAA的联合施用通过改变参与根生长、生长素运输和生物合成的几个基因的mRNA水平，改善了根拓扑系统和内源IAA含量。此外，根系氮代谢酶活性和氮相关基因表达水平的提高可能表明，葡萄糖和IAA的施用提高了硝酸盐（NO3—N）向氨基酸转化的速率。相反，单施TIBA降低了生长素转运基因的表达水平，阻碍了根系生长，降低了内源IAA含量。葡萄糖与TIBA联合施用有效地减弱了TIBA诱导的根拓扑结构、光合作用和N代谢活性以及参与生长素生物合成和运输的mRNA表达水平的降低。综上所述，葡萄糖施用可能改变了生长素合成和转运基因的表达水平，诱导了内源IAA在根系中的分配，从而改善了缺碳土壤中根系构型和根系氮代谢。
Sugars and auxin have important effects on almost all phases of plant life cycle, which are so fundamental to plants and regulate similar processes. However, little is known about the effect of cross-talk between glucose and indole-3-acetic acid (IAA) on growth and development of apple trees. To examine the potential roles of glucose and IAA in root architecture, root nitrogen (N) metabolism and photosynthetic capacity in ‘Hanfu’ (Malus domestica), a total of five treatments was established: single application of glucose, IAA, and auxin polar transport inhibitor (2, 3, 5-triiodobenzoic acid, TIBA), combined application of glucose with TIBA and that of glucose with IAA. The combined application of glucose with IAA improved root topology system and endogenous IAA content by altering the mRNA levels of several genes involved in root growth, auxin transport and biosynthesis. Moreover, the increased N metabolism enzyme activities and levels of genes expression related to N in roots may suggest higher rates of transformation of nitrate (NO3--N) into amino acids application of glucose and IAA. Contrarily, single application of TIBA decreased the expression levels of auxin transport gene, hindered root growth and decreased endogenous IAA content. Glucose combined with TIBA application effectively attenuated TIBA-induced reductions in root topology structure, photosynthesis and N metabolism activity, and mRNA expression levels involved in auxin biosynthesis and transport. Taken together, glucose application probably changes the expression level of auxin synthesis and transport genes, and induce the allocation of endogenous IAA in root, and thus improves root architecture and N metabolism of root in soil with deficit carbon.