Purification, secretion and immunocytochemical localization of the uterine milk proteins, major progesterone-induced proteins in uterine secretions of the sheep.
Purification, secretion and immunocytochemical localization of the uterine milk proteins, major progesterone-induced proteins in uterine secretions of the sheep.
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子宫乳蛋白（绵羊子宫分泌物中主要孕酮诱导蛋白）的纯化、分泌和免疫细胞化学定位。
DOI：
10.1095/biolreprod36.2.419
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发表时间：
1987
影响因子：
3.6
通讯作者：
Roberts,RM
中科院分区：
文献类型：
--
作者：
Moffatt,J;Bazer,FW;Hansen,PJ;Chun,PW;Roberts,RM
妊娠母羊将孕体限制在一个子宫角内，导致被称为子宫乳（UTM）的液体积聚在对侧子宫角内。两种基本多肽，称为子宫乳蛋白（UTM蛋白;通过聚丙烯酰胺凝胶电泳使用十二烷基硫酸钠测定，Mr= 55，000和57，000），占子宫乳中蛋白质的大部分。这两个UTM-蛋白质是糖蛋白，并且容易通过羧甲基（CM）-纤维素上的阳离子交换色谱法，然后通过Sephacryl S-200凝胶过滤从子宫液中纯化。通过平衡沉降分析测定，纯化的UIM蛋白质的重均分子量为50，700 ± 4，200。从怀孕的母羊子宫内膜外植体培养在放射性氨基酸的存在下，并释放到培养基中的UTM蛋白作为其主要分泌产物。UTM蛋白分泌到子宫腔的非妊娠，切除卵巢的母羊给予每日注射孕酮。雌酮单独是无效的诱导UTM蛋白的生产。免疫细胞化学研究表明，UTM蛋白的合成仅限于子宫的表面和腺上皮。
Restriction of the conceptus to one uterine horn of the pregnant ewe results in the accumulation of fluid called uterine milk (UTM) in the contralateral horn. Two basic polypeptides, called the uterine milk proteins (UTM-proteins; Mr= 55,000 and 57,000 as determined by polyacrylamide-gel electrophoresis using sodium dodecyl sulfate), accounted for the majority of the protein in uterine milk. The two UTM-proteins were glycoproteins and were readily purified from uterine fluids by cation-exchange chromatography on carboxymethyl (CM)-cellulose followed by Sephacryl S-200 gel-filtration. The purified UIM-proteins had a weight-average molecular weight of 50,700 ± 4,200, as determined by equilibrium sedimentation analysis. Endometrial explants from pregnant ewes were cultured in the presence of radioactive amino acids and released UTM-proteins into the medium as their major secretory products. The UTM-proteins were secreted into the uterine lumen of nonpregnant, ovariectomized ewes given daily injections of progesterone. Estrone alone was ineffective in inducing UTM-protein production. Immunocytochemical studies indicated that synthesis of the UTM-proteins was confined to the surface and glandular epithelium of the uterus.