HIV EXPRESSION STRATEGIES - RIBOSOMAL FRAMESHIFTING IS DIRECTED BY A SHORT SEQUENCE IN BOTH MAMMALIAN AND YEAST SYSTEMS
HIV EXPRESSION STRATEGIES - RIBOSOMAL FRAMESHIFTING IS DIRECTED BY A SHORT SEQUENCE IN BOTH MAMMALIAN AND YEAST SYSTEMS
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DOI：
10.1016/0092-8674(88)90260-7
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发表时间：
1988-12-23
期刊：
影响因子：
64.5
通讯作者：
KINGSMAN, AJ
中科院分区：
文献类型：
--
作者：
WILSON, W;BRADDOCK, M;KINGSMAN, AJ
人类免疫缺陷病毒（HIV-1）的pol基因表达为gag:pol融合，由核糖体移码引起，将重叠的、异相的gag和pol基因带入翻译阶段。在这项研究中，我们发现HIV的帧移位是由病毒RNA中的一个非常短的序列介导的。我们证明了同聚体在该序列中的重要性，并得出结论，HIV移码不依赖于移码位点下游的茎环结构。我们的分析还表明，在哺乳动物和酵母系统中，序列要求是相同的。
The pol gene of the human immunodeficiency virus (HIV-1) is expressed as a gag:pol fusion, arising from a ribosomal frameshift that brings the overlapping, out-of-phase gag and pol genes into translational phase. In this study, we show that HIV frameshifting is mediated by a very short sequence in the viral RNA. We demonstrate the importance of a homopolymeric run within this sequence and conclude that HIV frameshifting is not dependent on stem-loop structures downstream from the frameshift site. Our analysis also indicates that the sequence requirements are identical in mammalian and yeast systems.