Fine genotyping of a highly polymorphic ASTRINGENCY-linked locus reveals variable hexasomic inheritance in persimmon (Diospyros kaki Thunb.) cultivars
Fine genotyping of a highly polymorphic ASTRINGENCY-linked locus reveals variable hexasomic inheritance in persimmon (Diospyros kaki Thunb.) cultivars
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DOI：
10.1007/s11295-011-0432-0
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发表时间：
2012-02-01
影响因子：
2.4
通讯作者：
Yonemori, Keizo
中科院分区：
文献类型：
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作者：
Akagi, Takashi;Tao, Ryutaro;Yonemori, Keizo
柿子（Diospyros kaki Thunb.）是东亚地区主要的乔木作物之一，一般为六倍体。一个ASTRINGENCY （AST）基因座在6条对应的染色体上分别控制着柿子的涩味/非涩味（A/NA）性状，这是柿子最重要的食用性状之一。虽然一些分子方法正在研究柿子涩味性状的分子机制，但AST位点的独特多体行为仍有待解决。本研究的目的是对与AST位点相关的高多态性标记位点进行精细的基因分型，检测10个a型与na型品种杂交得到的F(1)系的等位基因配对，并确定AST位点的六倍体遗传基础。结果表明，柿子品种经常产生带有AST位点的额外染色体的非整倍体后代，不同品种间的非整倍体发生率不同。在对柿子品种六体行为的检验中，除cv外，从a型亲本分离得到的各等位基因的个体携带率与自六倍体遗传模型的预期比率吻合较好。符合自异源六倍体遗传模式的“罗天-天-石”和“沙谷-石”。这些结果表明柿子品种之间的六体体行为是可变的。
Persimmon (Diospyros kaki Thunb.) is one of the major tree crops in East Asia and is generally hexaploid. A single ASTRINGENCY (AST) locus controls the astringency/non-astringency (A/NA) trait of persimmon fruit, one of the most important traits for consumption, on each of the six corresponding chromosomes. Although several molecular approaches are in progress to elucidate the molecular mechanisms of astringency trait in persimmon, the distinct polysomic behavior of the AST locus remains to be solved. The aim of this study was to perform fine genotyping of a highly polymorphic marker locus linked to the AST locus, detect the allele pairing in ten segregated F(1) lines derived from hybridization of A-type x NA-type cultivars, and identify the basis of hexaploid inheritance at the AST locus in persimmon. The results showed that persimmon cultivars frequently produce aneuploid offspring bearing an extra chromosome with the AST locus, with the incidence of aneuploidy varying among the cultivars. On the examination of hexasomic behavior in persimmon cultivars, the ratios of individuals bearing each allele pair segregated from A-type parents showed a good fit to the expected ratios in an autohexaploid inheritance model, except for cvs. Luo-tian-tian-shi and Sa-gok-shi which fitted to an autoallohexaploid inheritance model. These results suggest variable hexasomic behavior among persimmon cultivars.