Preparation of Lactose-Modified Cellulose Films by a Nonaqueous Enzymatic Reaction and their Biofunctional Characteristics as a Scaffold for Cell Culture
Preparation of Lactose-Modified Cellulose Films by a Nonaqueous Enzymatic Reaction and their Biofunctional Characteristics as a Scaffold for Cell Culture
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DOI：
10.1021/bm900089j
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发表时间：
2009-05-01
影响因子：
6.2
通讯作者：
Wariishi, Hiroyuki
中科院分区：
文献类型：
--
作者：
Esaki, Kei;Yokota, Shingo;Wariishi, Hiroyuki
采用非水相生物催化法对透明纤维素膜进行乳糖糖基化修饰，并研究了大鼠肝细胞在乳糖修饰纤维素膜上的粘附行为。使用表面活性剂包封的纤维素酶在氯化锂/二甲基乙酰胺溶剂中在37 ° C下将再生的纤维素膜与乳糖孵育24小时，并且将乳糖分子成功地引入到纤维素膜中。乳糖修饰的纤维素膜上的初始细胞粘附优于原始纤维素膜的上级。在不存在血清的情况下，肝细胞仅显著附着在乳糖修饰的纤维素膜上。这一过程被作为抑制剂的游离乳糖的加入显着抑制。这些结果表明，这种细胞粘附进行通过纤维素膜上的半乳糖残基和大鼠肝细胞表面上的去唾液酸糖蛋白受体之间的直接相互作用。这种新型的纤维素基质表面糖基化修饰方法及其生物功能特性有望作为细胞培养工程的生物活性支架提供潜在的应用。
Enzymatic glyco-modification of transparent cellulose films with lactose was achieved by nonaqueous biocatalysis, and rat hepatocyte attachment behavior to the lactose-modified cellulose films was investigated. Regenerated cellulose films were incubated with lactose using a surfactant-enveloped cellulase in lithium chloride/dimethylacetamide solvent at 37 degrees C for 24 h, and lactose molecules were successfully introduced to the cellulose films. The initial cell adhesion on the lactose-modified cellulose films was superior to the original cellulose film. In the absence of serum, hepatocytes were significantly attached only on the lactose-modified cellulose films. This process was markedly suppressed by the addition of free lactose as an inhibitor. These results suggest that such cell adhesion proceeded through a direct interaction between galactose residues on the cellulose films and asialoglycoprotein receptors on the rat liver cell surface. This novel approach for surface glyco-modification of a cellulose matrix and its biofunctional properties are expected to provide potential application as a bioactive scaffold for cell culture engineering.