Influential Factors of the Indeterminate Results Tested by QuantiFERON-TB Gold In-Tube (QFT-IT) Assay for Diagnosing TB Infection in HIV-Infected Patients
Influential Factors of the Indeterminate Results Tested by QuantiFERON-TB Gold In-Tube (QFT-IT) Assay for Diagnosing TB Infection in HIV-Infected Patients
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用于诊断 HIV 感染者结核感染的 QuantiFERON-TB 金管内 (QFT-IT) 检测不确定结果的影响因素
DOI：
10.7754/clin.lab.2016.160209
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发表时间：
2016-01-01
影响因子：
0.7
通讯作者：
Hou, Wei
中科院分区：
文献类型：
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作者：
Dai, Qinghai;Qiao, Kunyan;Hou, Wei
背景：QuantiFERON-TB Gold试管内检测（QFT-IT）是诊断HIV感染患者结核病的推荐方法。然而，不确定结果的发生率高于未感染HIV的患者。我们前期的研究表明，CD 4细胞计数是QFT-IT不确定结果的重要影响因素，其他影响因素是否影响QFT-IT结果尚不清楚。本文对98例HIV感染者可疑结核病感染者进行外周血QFT-IT检测，探讨其与CD 4 T细胞计数、淋巴细胞计数、淋巴细胞百分比、结果：有发热、排痰等临床症状，经痰培养、胸片等检查确诊的8例患者，均为QFT阳性（阳性率100%）。其余90例无临床症状的患者检测为阴性（n = 51），不确定（n = 33）和阳性（n = 6）。阳性组和阴性组的淋巴细胞计数、淋巴细胞百分比和CD 4 T细胞计数均显著高于不确定组（均p < 0.05）。结论：QFT-IT可能是一种潜在的、简单可行的方法。艾滋病毒感染者结核病的诊断和筛查。此外，CD 4 T细胞计数、淋巴细胞计数和淋巴细胞百分比是QFT-IT不确定结果的影响因素。
Background: QuantiFERON-TB Gold In-Tube test (QFT-IT) is a recommended method for diagnosing TB in HIV-infected patients. However, the rate of indeterminate results is higher than that in HIV-uninfected patients. Our previous study showed that the CD4 cell count was an important influential factor for the indeterminate results of QFT-IT. Whether other influential factors affect the QFT-IT results was unclear.Methods: In this paper, 98 HIV-infected patients with suspected TB infection were enrolled and the plasma of peripheral blood was collected for QFT-IT. The relationship between the CD4 T-cell count, lymphocyte count, lymphocyte percentage, and the results of QFT-IT were analyzed.Results: All patients who have the clinical symptoms of TB including fever and productive cough and were later confirmed by other assays including the sputum culture and chest radiography (n = 8) tested as QFT-positive (positive rate 100%). The other 90 patients without clinical symptoms tested as negative (n = 51), indeterminate (n = 33), and positive (n = 6). The lymphocyte count and the lymphocyte percentage as well as the CD4 T-cell count were significant higher in the positive group and negative group than in the indeterminate group (p < 0.05, for all).Conclusions: The QFT-IT could be a potential, simple, and feasible method for diagnosing and screening TB in HIV-infected patients. In addition, the CD4 T-cell count, the lymphocyte count, and the lymphocyte percentage were influential factors of the indeterminate results of QFT-IT.