IMPROVEMENT OF CYTOTOXICITY OF TUMOR-NECROSIS-FACTOR (TNF) BY INCREASE IN BASICITY OF ITS N-TERMINAL REGION
IMPROVEMENT OF CYTOTOXICITY OF TUMOR-NECROSIS-FACTOR (TNF) BY INCREASE IN BASICITY OF ITS N-TERMINAL REGION
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DOI：
10.1016/0006-291x(87)90923-5
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发表时间：
1987-10-29
影响因子：
3.1
通讯作者：
MIZUNO, D
中科院分区：
文献类型：
--
作者：
SOMA, GI;KITAHARA, N;MIZUNO, D
Two new recombinant TNFs (named rTNF-Scw1 and -Scw2) with higher basicity than conventional recombinant human TNF- .alpha. (rTNF- .alpha.) in the N-terminal region were constructed. Their sequences were constructed based on those of partially purified cytotoxic factors from the culture supernatant of acute monocytic leukemia cells THP-1, which unlike rTNF- .alpha. are cytotoxic to T24 bladder carcinoma cells in vitro. These new rTNF-Ss showed a broader cytotoxicity to tumor cells than rTNF- .alpha.. This increase in the basicity of the N-terminal region over that of conventional TNF significantly increased the cytotoxicity on tumor cells in vivo as well as in vitro.
