Antifungal effect and mechanism of garlic oil on Penicillium funiculosum
Antifungal effect and mechanism of garlic oil on Penicillium funiculosum
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DOI：
10.1007/s00253-014-5919-9
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发表时间：
2014-10-01
影响因子：
5
通讯作者：
Chen, Yi-Ben
中科院分区：
文献类型：
--
作者：
Li, Wen-Ru;Shi, Qing-Shan;Chen, Yi-Ben
大蒜油是一种杀菌剂，但其抑菌效果及作用机制尚不清楚。以绳状青霉（Penicillium funculosum）为模型菌株，研究了大蒜油的化学成分、抑菌活性及其作用。结果表明，大蒜油在琼脂培养基和肉汤培养基中的最低抑菌浓度（MFCs，v/v）分别为0.125%和0.0313%，表明大蒜油具有较强的抑菌活性。经气相色谱-质谱联用（GC/MS）分析，大蒜油的主要成分为硫化物，其中二硫化物（36%）、三硫化物（32%）和单硫化物（29%）是大蒜油抑菌的主要成分。透射电镜（TEM）和扫描电镜（SEM）观察结果表明，大蒜油首先渗入菌丝细胞，甚至细胞器，破坏细胞结构，最终导致细胞质和大分子物质的渗漏。进一步的蛋白质组学分析表明，大蒜油能够诱导一些生理代谢关键蛋白的表达增加或减弱。因此，我们的研究证明大蒜油可以作用于绳状青霉菌丝的多个部位，导致其死亡。大蒜油具有良好的抑菌效果，在抗真菌工业中具有广阔的应用前景。
Garlic oil is a kind of fungicide, but little is known about its antifungal effects and mechanism. In this study, the chemical constituents, antifungal activity, and effects of garlic oil were studied with Penicillium funiculosum as a model strain. Results showed that the minimum fungicidal concentrations (MFCs, v/v) were 0.125 and 0.0313 % in agar medium and broth medium, respectively, suggesting that the garlic oil had a strong antifungal activity. The main ingredients of garlic oil were identified as sulfides, mainly including disulfides (36 %), trisulfides (32 %) and monosulfides (29 %) by gas chromatograph-mass spectrometer (GC/MS), which were estimated as the dominant antifungal factors. The observation results by transmission electron microscope (TEM) and scanning electron microscope (SEM) indicated that garlic oil could firstly penetrate into hyphae cells and even their organelles, and then destroy the cellular structure, finally leading to the leakage of both cytoplasm and macromolecules. Further proteomic analysis displayed garlic oil was able to induce a stimulated or weakened expression of some key proteins for physiological metabolism. Therefore, our study proved that garlic oil can work multiple sites of the hyphae of P. funiculosum to cause their death. The high antifungal effects of garlic oil makes it a broad application prospect in antifungal industries.