Basal lamina development in chicken muscle spindles.
Basal lamina development in chicken muscle spindles.
复制标题
鸡肌梭的基底层发育。
DOI：
10.1002/aja.1002020307
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Mayne,R
中科院分区：
文献类型：
--
作者：
Maier,A;Mayne,R
用免疫组织化学方法观察了鸡胚13 ~ 8周肌梭基底层的发育。层粘连蛋白同种型免疫染色可见基底层粘连的片段已在E6肌肉中观察到。当在E13 - 14首次识别梭内肌管簇时，它们被基底层包围，基底层在覆盖和分子组成方面都不完整。在E18，更成熟的基膜管单独包围年轻的肌纤维。在与肌管接触后，肌感觉连接处的基底层的突触部分与抗s-层粘连蛋白和硫酸软骨素蛋白聚糖的抗体反应强烈，而突触外部分为阴性或仅反应微弱。在神经肌肉接头的突触基底层，硫酸肝素蛋白聚糖和s-层粘连蛋白在E16后变得突出。与梭内纤维周围基底层蛋白的早期存在相反，直到E17 - 18才发现外梭囊中基底层蛋白的初始沉积，并且直到出生后第1周才检测到显著量。与梭内基底层不同，囊基底层没有明显的专门区域，然而，梭内和囊基底层的分子组成相似。© 1995 Wiley利斯公司
The development of basal laminas was examined in immunohistochemical sections of chicken leg muscle spindles from embryonic day (E) 13 to 8 weeks postnatal. Fragments of basal laminas as seen with immunostaining for isoforms of laminin were already observed in E6 muscles. When clusters of intrafusal myotubes were first recognized at E13‐14, they were surrounded by basal laminas which were incomplete both in terms of coverage and molecular composition. More mature basal lamina tubes individually enclosed young myofibers at E18. Afterents made contact with myotubes, synaptic portions of basal laminas at myosensory junctions reacted strongly with antibodies against s‐laminin and chondroitin sulfate proteoglycan, while extrasynaptic portions were negative or reacted only weakly. At synaptic basal laminas of neuromuscular junctions heparin sulfate proteoglycan and s‐laminin became prominent after E16. Contrary to the early presence of basal lamina proteins around intrafusal fibers, initial deposition of basal lamina proteins in the outer spindle capsule was not recognized until E17‐18, and significant amounts were not detected until postnatal week 1. Unlike intrafusal basal laminas, capsular basal laminas developed no distinct specialized regions; however, molecular compositions of intrafusal and capsular basal laminas were similar. © 1995 Wiley‐Liss, Inc.