Rational Design of Novel Lipophilic Aggregation-Induced Emission Probes for Revealing the Dynamics of Lipid Droplets during Lipophagy and Ferroptosis
Rational Design of Novel Lipophilic Aggregation-Induced Emission Probes for Revealing the Dynamics of Lipid Droplets during Lipophagy and Ferroptosis
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DOI：
10.1021/acs.analchem.2c02260
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发表时间：
2022-09-19
影响因子：
7.4
通讯作者：
Li, Yinhui
中科院分区：
文献类型：
--
作者：
Chen, Rui;Li, Zilu;Li, Yinhui
脂滴作为重要的细胞器，在某些生理过程中发挥着重要作用。在某些生理过程中，监测LD的浓度以及LD与其他细胞器之间的动态行为，对于研究LD的生物学功能和医学诊断具有重要意义。本文报道了一系列基于喹啉丙二腈（QM）和咔唑偶联的聚集诱导发射（AIE）探针AIE-CbzLD-Cn（n = 1，3，5，7，OMe），用于跟踪LD的动态变化，研究LD与溶酶体/内质网（ER）的结合。AIE-Cbz-LD-C-3、AIE-Cbz-LD-C-5和AIE-Cbz-LD-C-7能够准确地聚集在LD中，并具有良好的光稳定性。其中，AIE-Cbz-LD-C-7由于出色的LD特异性而用于可视化在脂肪吞噬期间LD与溶酶体之间的相互作用。我们还成功地跟踪了内质网区域附近产生的新生LD的数量，揭示了通过使用AIE-Cbz-LD-C-7，在铁凋亡期间，该数量显著增加，这为LD从ER产生的假设提供了有用的证据。我们希望AIE-Cbz-LD-C-7探针能够成为一种实用的工具，用于跟踪LD接触的生理和病理过程。
Lipid droplets (LDs), as crucial organelles, play a significant role in some physiological processes. Monitoring the concentration of LDs and dynamic behaviors between LDs and other organelles during some physiological processes is important for studying their biological function and medical diagnosis. Herein, we report a series of aggregation-induced emission (AIE) probes AIE-CbzLD-Cn (n = 1, 3, 5, 7, OMe) based on the conjugation of quinolinemalononitrile (QM) and carbazole for tracking the dynamic changes of LDs and studying the association between LDs and lysosome/ endoplasmic reticulum (ER). To our great delight, AIE-Cbz-LD-C-3, AIE-Cbz-LD-C-5, and AIE-Cbz-LD-C-7 could aggregate in LDs accurately and light up the LDs with good photostability. Among them, AIE-Cbz-LD-C-7 was used to visualize the interplay between LDs and lysosomes during lipophagy due to the excellent LD-specificity. We also succeeded in tracking the number of newborn LDs generated near the endoplasmic reticulum regions revealing that the number increased considerably during ferroptosis by using AIE-Cbz-LD-C-7, which supplies useful evidence for the hypothesis that LDs generate from the ER. We expect the probe AIE-Cbz-LD-C-7 would be a practical tool for tracking the physiological and pathological processes contacted with LDs.