Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity
Sequencing wild and cultivated cassava and related species reveals extensive interspecific hybridization and genetic diversity
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DOI：
10.1038/nbt.3535
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发表时间：
2016-05-01
影响因子：
46.9
通讯作者：
Rokhsar, Daniel S.
中科院分区：
文献类型：
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作者：
Bredeson, Jessen V.;Lyons, Jessica B.;Rokhsar, Daniel S.
木薯（Manihot esculenta）为5亿多人提供热量和营养。它是由亚马逊土著人通过种植野生祖先M。可食草flabellifolia，现在生长在世界各地的热带地区。在这里，我们提供了一个高质量的木薯基因组组装与改进的连续性，连锁和完整性;几乎97%的基因锚定到染色体。我们发现，木薯的古四倍体是共享的相关橡胶树橡胶树，提供了资源进行比较研究。我们还对全球收集的58个木薯种质进行了测序，包括栽培和野生木薯种质以及相关物种，如塞阿拉或印度橡胶（M. glaziovii）和基因型268个非洲木薯品种。我们发现广泛的种间混合物，并检测过去木薯育种计划的遗传特征。木薯作为一种无性繁殖作物，特别容易受到病原体和非生物胁迫的影响。这种基因组资源将为未来的基因组育种工作提供信息，以改善这种主要作物。
Cassava (Manihot esculenta) provides calories and nutrition for more than half a billion people. It was domesticated by native Amazonian peoples through cultivation of the wild progenitor M. esculenta ssp. flabellifolia and is now grown in tropical regions worldwide. Here we provide a high-quality genome assembly for cassava with improved contiguity, linkage, and completeness; almost 97% of genes are anchored to chromosomes. We find that paleotetraploidy in cassava is shared with the related rubber tree Hevea, providing a resource for comparative studies. We also sequence a global collection of 58 Manihot accessions, including cultivated and wild cassava accessions and related species such as Ceara or India rubber (M. glaziovii), and genotype 268 African cassava varieties. We find widespread interspecific admixture, and detect the genetic signature of past cassava breeding programs. As a clonally propagated crop, cassava is especially vulnerable to pathogens and abiotic stresses. This genomic resource will inform future genome-enabled breeding efforts to improve this staple crop.