Cutting edge: in vitro generated Th17 cells maintain their cytokine expression program in normal but not lymphopenic hosts.
Cutting edge: in vitro generated Th17 cells maintain their cytokine expression program in normal but not lymphopenic hosts.
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DOI：
10.4049/jimmunol.0803931
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发表时间：
2009-03-01
期刊：
影响因子：
--
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Nurieva R;Yang XO;Chung Y;Dong C
激活后，初始 CD4+ T 细胞分化为效应 Th 细胞亚群。效应 Th 细胞的稳定性和可塑性尚未得到充分了解。在本研究中，我们使用IL-17F-红色荧光蛋白报告小鼠来分析Th17细胞的体外和体内可塑性。我们发现体外产生的 Th17 细胞在体外很难维持其分化程序，并且可以被重新编程为其他 T 细胞谱系。此外，在转移到淋巴细胞减少的宿主中后，Th17细胞迅速失去IL-17表达，并转化为独立于IL-7信号传导的Th1细胞。然而，即使在没有 Ag 和炎症的情况下，Th17 细胞在正常动物中也能很好地保持其表型。这些结果虽然表明 Th17 细胞具有可塑性，但也表明其程序在体内的积极维持。
Upon activation, naive CD4+ T cells differentiate into effector Th cell subsets. The stability and plasticity of effector Th cells have not been well understood. In this study we used an IL-17F-red fluorescent protein reporter mouse to analyze the plasticity of Th17 cells in vitro and in vivo. We found that in vitro generated Th17 cells poorly maintained their differentiation program in vitro and could be reprogrammed into other T cell lineages. Moreover, upon transfer into lymphopenic hosts, Th17 cells rapidly lost their IL-17 expression and were converted into Th1 cells independently of IL-7 signaling. However, Th17 cells maintained their phenotypes well in normal animals, even in the absence of Ag and inflammation. These results, although suggesting the plasticity of Th17 cells, also indicate active maintenance of their program in vivo.
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