A phylogenomic and molecular marker based proposal for the division of the genus Borrelia into two genera: the emended genus Borrelia containing only the members of the relapsing fever Borrelia, and the genus Borreliella gen. nov containing the members of the Lyme disease Borrelia (Borrelia burgdorferi sensu lato complex)
A phylogenomic and molecular marker based proposal for the division of the genus Borrelia into two genera: the emended genus Borrelia containing only the members of the relapsing fever Borrelia, and the genus Borreliella gen. nov containing the members of the Lyme disease Borrelia (Borrelia burgdorferi sensu lato complex)
复制标题
DOI：
10.1007/s10482-014-0164-x
复制
发表时间：
2014-06-01
影响因子：
2.6
通讯作者：
Gupta, Radhey S.
中科院分区：
文献类型：
--
作者：
Adeolu, Mobolaji;Gupta, Radhey S.
伯氏疏螺旋体属包含两组生物体：莱姆病病原体及其亲属和回归热病原体及其亲属。这两组在形态上难以区分，在生物化学上也难以区分。在这项工作中，我们对来自38个伯氏疏螺旋体基因组的蛋白质序列进行了详细的比较基因组分析，以确定在伯氏疏螺旋体同源物中特异性存在的保守特征插入/缺失（CSIs）形式的分子标记，以及在伯氏疏螺旋体物种中独特存在的保守特征蛋白（CSPs）。我们的分析已经确定了31个CSIs和82个csp，这些csp是所有测序的伯氏疏螺旋体物种所独有的，为这类生物提供了分子标记。此外，我们的工作已经确定了7个CSIs和21个CSPs，这是莱姆病伯氏疏螺旋体物种特有的，8个CSIs和4个CSPs是回归热伯氏疏螺旋体群成员特有的。此外，在伯氏疏螺旋体中发现的蛋白质中的38个其他CSIs也可以区分这两组伯氏疏螺旋体。所鉴定的CSIs和CSPs为莱姆病伯氏疏螺旋体和回归热伯氏疏螺旋体的鉴定和区分提供了新的、高度特异性的分子标记。我们还报道了伯氏疏螺旋体基因组的平均核苷酸同源性（ANI）分析结果，以及基于25个保守蛋白的16S rRNA序列和串联序列的系统发育分析结果。这些分析也支持了两个疏螺旋体分支的独特性。基于已鉴定的分子标记、ANI和系统发育研究的结果，以及不同伯氏疏螺旋体传播的不同致病性特征和节肢动物媒介，我们建议将伯氏疏螺旋体划分为两个独立的属：包含回归热病原体的修正属伯氏疏螺旋体和包含莱姆病病原体的新属伯氏疏螺旋体。
The genus Borrelia contains two groups of organisms: the causative agents of Lyme disease and their relatives and the causative agents of relapsing fever and their relatives. These two groups are morphologically indistinguishable and are difficult to distinguish biochemically. In this work, we have carried out detailed comparative genomic analyses on protein sequences from 38 Borrelia genomes to identify molecular markers in the forms of conserved signature inserts/deletions (CSIs) that are specifically found in the Borrelia homologues, and conserved signature proteins (CSPs) which are uniquely present in Borrelia species. Our analyses have identified 31 CSIs and 82 CSPs that are uniquely shared by all sequenced Borrelia species, providing molecular markers for this group of organisms. In addition, our work has identified 7 CSIs and 21 CSPs which are uniquely found in the Lyme disease Borrelia species and eight CSIs and four CSPs that are specific for members of the relapsing fever Borrelia group. Additionally, 38 other CSIs, in proteins which are uniquely found in Borrelia species, also distinguish these two groups of Borrelia. The identified CSIs and CSPs provide novel and highly specific molecular markers for identification and distinguishing between the Lyme disease Borrelia and the relapsing fever Borrelia species. We also report the results of average nucleotide identity (ANI) analysis on Borrelia genomes and phylogenetic analysis for these species based upon 16S rRNA sequences and concatenated sequences for 25 conserved proteins. These analyses also support the distinctness of the two Borrelia clades. On the basis of the identified molecular markers, the results from ANI and phylogenetic studies, and the distinct pathogenicity profiles and arthropod vectors used by different Borrelia spp. for their transmission, we are proposing a division of the genus Borrelia into two separate genera: an emended genus Borrelia, containing the causative agents of relapsing fever and a novel genus, Borreliella gen. nov., containing the causative agents of Lyme disease.