Expression of cathepsin L in human testis under diverse infertility conditions
Expression of cathepsin L in human testis under diverse infertility conditions
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DOI：
10.1080/01485010490425223
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发表时间：
2004-05-01
期刊：
影响因子：
--
通讯作者：
Kim, ST
中科院分区：
文献类型：
--
作者：
Gye, MC;Kim, ST
组织蛋白水解酶家族在啮齿类动物睾丸生精上皮重塑中起重要作用。为了揭示组织蛋白酶L在不同病理条件下睾丸组织中的表达，采用免疫组织化学方法对不同男性不育条件下睾丸组织中组织蛋白酶L的表达进行了研究。在正常、非梗阻性无精子症、生精功能减退和成熟停滞的生精小管中，组织蛋白酶L在生殖细胞和支持细胞中均有表达。相反，在仅支持细胞综合征、生精细胞发育不全和睾丸癌的生精小管组织中，组织蛋白酶L没有明显的表达。我们的结果表明，生殖细胞和支持细胞之间的相互作用对组织蛋白酶L在人睾丸中的表达起着关键的调控作用。组织蛋白酶L在生殖细胞癌中的表达缺失，说明组织蛋白酶L在支持细胞中的表达受人睾丸功能生殖细胞的调控。
The cathepsin family of proteolytic enzymes play an important role in the remodeling seminiferous epithelia in rodent testis. In an effort to uncover the cathepsin L expression in diverse pathological conditions in human testis, the immunohistochemical localization of cathepsin L was conducted in human testis under diverse male infertility condition including spermatogenic hypoplasia and testis cancer. In seminiferous tubule of normal, non-obstructive azoospermic, decreased spermatogenesis, and maturation arrest conditions, cathepsin L was found in both germ cells and Sertoli cells. In contrast, there was no visible expression of cathepsin L in seminiferous tubule tissues from Sertoli cell-only syndrome, spermatogenic hypoplasia, and testicular cancer. Our result suggests that the cross-talk between germ cells and Sertoli cells is crucial for the control of cathepsin L expression in human testis. The absence of expression of cathepsin L in germ cell cancer emphasizes that cathepsin L expression in Sertoli cells is regulated by functional germ cells in human testis.