Single-Scan Selective Excitation of Individual NMR Signals in Overlapping Multiplets
Single-Scan Selective Excitation of Individual NMR Signals in Overlapping Multiplets
复制标题
重叠多重峰中单个 NMR 信号的单扫描选择性激发
DOI：
10.1002/ange.202011642
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发表时间：
2020
影响因子：
--
通讯作者：
Kiraly P
中科院分区：
文献类型：
--
作者：
Kiraly P
2D核磁共振是一种非常强大的结构工具，但它很耗时。在一维实验中，通过选择性激发来锁定单个化学基团可以更快地提供所需的信息。然而，一个主要的问题是，质子核磁共振谱经常广泛重叠，因此在实践中只有少数位置可以选择性地激发。在这里，我们使用一种快速的单扫描方法克服了这个问题，这种方法允许选择性地激发单个质子多重态的信号，即使它与其他多重态严重重叠。该方法的优点在选择性的一维NOESY实验中得到了说明，这是通过核磁共振明确确定相对构型的最有效的方法。这里提出的新方法有可能极大地扩大选择性激发的适用性，并为许多其他实验释放其真正的潜力。
2D NMR is an immensely powerful structural tool but it is time‐consuming. Targeting individual chemical groups by selective excitation in a 1D experiment can give the information required far more quickly. A major problem, however, is that proton NMR spectra are often extensively overlapped, so that in practice only a minority of sites can be selectively excited. Here we overcome that problem using a fast, single‐scan method that allows selective excitation of the signals of a single proton multiplet even where it is severely overlapped by other multiplets. The advantages of the method are illustrated in a selective 1D NOESY experiment, the most efficient way to determine relative configuration unambiguously by NMR. The new approach presented here has the potential to broaden significantly the applicability of selective excitation and unlock its real potential for many other experiments.