CALCIUM BINDING, QUANTUM YIELD, AND EMITTING MOLECULE IN AEQUORIN BIOLUMINESCENCE
CALCIUM BINDING, QUANTUM YIELD, AND EMITTING MOLECULE IN AEQUORIN BIOLUMINESCENCE
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DOI：
10.1038/2271356a0
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发表时间：
1970-01-01
期刊：
影响因子：
64.8
通讯作者：
JOHNSON, FH
中科院分区：
文献类型：
--
作者：
SHIMOMURA, O;JOHNSON, FH
水母中的蛋白Aequorin在水溶液中发出蓝光，特别是在钙或锶的作用下；在峰值波长上所需的大于60千卡/摩尔的能量明显是由分子内反应提供的，并伴随着吸收光谱的变化，产生蓝色荧光反应产物1，2。对Ca~(2+)的极端敏感性，比对Sr2+更敏感，为快速测定生物液体中的Ca~(2+)提供了基础，并已被用于检测Ca~(2+)与单个肌肉纤维4，5的收缩和线粒体6的活性的关系。研究了水飞蓟素的主要性质，包括相对分子质量(M.W.)据报告，其中30,000个是通过沉淀、扩散或32,000个氨基酸组成7。对发光反应的动力学也进行了研究。
THE protein aequorin, from the jellyfishAequorea, emits bluish light in aqueous solution on addition specifically of calcium or strontium; the necessary energy of more than 60 kcalories/mole at the peak wavelength is evidently provided by an intramolecular reaction which is accompanied by a change in absorption spectrum and yields a blue fluorescent reaction product1,2. The extreme sensitivity to Ca2+, much more than to Sr2+, provides a basis for quick microdetermination of Ca2+in biological fluids3and has been used in detecting the relation of Ca2+to contraction of single muscle fibres4,5and to activity of mitochondria6. The chief properties of aequorin, including the molecular weight (m.w.) of 30,000 by sedimentation, diffusion or 32,000 by amino-acid composition, have been reported7. The kinetics of the light-emitting reaction have also been studied8.