Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae
Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae
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DOI：
10.1111/j.1567-1364.2007.00302.x
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发表时间：
2008-02-01
影响因子：
3.2
通讯作者：
Jewett, Michael C.
中科院分区：
文献类型：
--
作者：
Nielsen, Jens;Jewett, Michael C.
工业生物技术是一个快速发展的领域。随着越来越多的人转向以生物为基础的经济，人们对发展高效的细胞工厂的需求不断增加，这些工厂可以生产燃料、化学品、药品、材料、营养食品，甚至食品配料。酿酒酵母非常适合这一目标。作为研究最深入的真核模式生物之一，丰富的知识密度详细介绍了它的遗传学、生物化学、生理学和大规模发酵性能，可以利用它来大幅增加这种酵母的工业应用。基因组学和高通量系统生物学工具的发展正在提高人们快速描述细胞行为的能力，这在代谢工程领域是有价值的，因为在代谢工程领域，菌株表征往往是菌株开发计划的瓶颈。本文综述了系统生物学对代谢工程的影响，并对系统生物学在细胞工厂设计中的作用进行了展望。
Industrial biotechnology is a rapidly growing field. With the increasing shift towards a bio-based economy, there is rising demand for developing efficient cell factories that can produce fuels, chemicals, pharmaceuticals, materials, nutraceuticals, and even food ingredients. The yeast Saccharomyces cerevisiae is extremely well suited for this objective. As one of the most intensely studied eukaryotic model organisms, a rich density of knowledge detailing its genetics, biochemistry, physiology, and large-scale fermentation performance can be capitalized upon to enable a substantial increase in the industrial application of this yeast. Developments in genomics and high-throughput systems biology tools are enhancing one's ability to rapidly characterize cellular behaviour, which is valuable in the field of metabolic engineering where strain characterization is often the bottleneck in strain development programmes. Here, the impact of systems biology on metabolic engineering is reviewed and perspectives on the role of systems biology in the design of cell factories are given.