Revealing the process of storage protein rebalancing in high quality protein maize by proteomic and transcriptomic
Revealing the process of storage protein rebalancing in high quality protein maize by proteomic and transcriptomic
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DOI：
10.1016/j.jia.2022.08.031
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发表时间：
2023-05-11
影响因子：
4.8
通讯作者：
Qiu, Fa-Zhan
中科院分区：
文献类型：
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作者：
Zhao, Hai-Liang;Qin, Yao;Qiu, Fa-Zhan
优质蛋白玉米 (QPM) (Zea mays L.) 品种含有更高水平的色氨酸和赖氨酸，对人类和牲畜表现出更高的营养价值。然而，由于重新平衡储存蛋白的过程复杂，育种 QPM 品种仍然具有挑战性。本研究进行了转录组和蛋白质组分析，以研究 opaque2 (o2) 和 QPM 中储存蛋白重新平衡的过程。我们发现转录组和蛋白质组之间的相关性较弱，这表明转录后事件对 Mo17o2 和 QPM 中的非玉米醇溶蛋白丰度具有显着调节作用。这些结果凸显了蛋白质组学的优势。与Mo17相比，Mo17o2和QPM中均鉴定出672个差异表达蛋白（DEP），其中一些与储存蛋白、淀粉和氨基酸合成相关。我们在 Mo17o2 和 QPM 籽粒中鉴定出 178 种非玉米醇溶蛋白作为 DEP。上调的非玉米醇溶蛋白 DEP 富含赖氨酸、色氨酸和蛋氨酸，这影响了蛋白质质量。共表达网络分析确定了 QPM 中储存蛋白合成的调节因子，包括 O2、PBF1 和几种转录因子。我们的结果揭示了储存蛋白重新平衡是如何发生的，并确定了可能促进优质 QPM 育种的非玉米醇溶蛋白 DEP。
Quality protein maize (QPM) (Zea mays L.) varieties contain enhanced levels of tryptophan and lysine, exhibiting improved nutritive value for humans and livestock. However, breeding QPM varieties remains challenging due to the complex process of rebalancing storage protein. This study conducted transcriptome and proteome analyses to investigate the process of storage proteins rebalancing in opaque2 (o2) and QPM. We found a weak correlation between the transcriptome and proteome, suggesting a significant modulating effect of post-transcriptional events on non-zein protein abundances in Mo17o2 and QPM. These results highlight the advantages of proteomics. Compared with Mo17, 672 differentially expressed proteins (DEPs) were identified both in Mo17o2 and QPM, and several of them were associated with storage protein, starch, and amino acid synthesis. We identified 178 non-zeins as DEPs in Mo17o2 and QPM kernels. The up-regulated non-zein DEPs were enriched in lysine, tryptophan, and methionine, which affected the protein quality. Co-expression network analysis identified regulators of storage protein synthesis in QPM, including O2, PBF1, and several transcription factors. Our results revealed how storage protein rebalancing occurs and identified nonzein DEPs that may facilitate superior-quality QPM breeding.