Inhibition of calcium channel activation in GH3 cells by static magnetic fields
Inhibition of calcium channel activation in GH3 cells by static magnetic fields
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DOI：
10.1016/0005-2736(96)00053-3
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发表时间：
1996-06-13
影响因子：
3.4
通讯作者：
Rosen, AD
中科院分区：
文献类型：
--
作者：
Rosen, AD
用全细胞膜片钳技术检测培养的GH3细胞的电压激活钙通道功能。暴露在120mT的恒磁场中，钙电流峰值幅度略有降低，电流-电压关系发生了漂移。最显著的变化是通道激活率减慢，而失活率没有任何变化。通道功能中的所有机会都是可逆的，在关闭磁场后3分钟内恢复到曝光前的值。这些通道功能的改变是温度依赖性的。目前的发现与磁诱导的膜变形对钙通道膜内部分的功能破坏是一致的。
Voltage activated calcium channel function was examined in cultured GH3 cells using the whole-cell patch clamp technique. Exposure to a 120 mT static magnetic field resulted in a slight reduction in the peak calcium current amplitude and shift in the current-voltage relationship. The most significant change was a slowing of the channel activation rate without any change in the inactivation rate. All chances in channel function were reversible, with return to pre-exposure values within 3 min after the field was turned off. These alterations in channel function were temperature-dependent. The present findings are consistent with a functional disruption of the intramembranous portion of the calcium channel by a magnetically induced membrane deformation.