Fabrication of two distinct hydroxyapatite coatings and their effects on MC3T3-E1 cell behavior
Fabrication of two distinct hydroxyapatite coatings and their effects on MC3T3-E1 cell behavior
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两种不同羟基磷灰石涂层的制备及其对 MC3T3-E1 细胞行为的影响
DOI：
10.1016/j.colsurfb.2018.06.046
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发表时间：
2018-11-01
影响因子：
5.8
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Pang, Shumin;He, Yuan;Li, Hong
羟基磷灰石（HAP）涂层的表面形貌如何重塑硬组织是最重要的，也是导致这一主题模糊的一个因素。本文通过控制电解质浓度，采用水热电沉积的方法在Ti衬底上合成了具有c轴取向的棒状纳米阵列和a(b)轴取向的片状微花阵列两种不同形貌的HAP涂层。XRD、TEM和SAED分析表明，棒状HAP纳米阵列以（001）取向为主，而HAP微花样品以（100）取向为主。与片状HAP相比，棒状HAP具有更好的亲水性和更低的粗糙度，不仅增强了特异性纤维连接蛋白的吸附，而且促进了MC3T3-E1细胞的扩散和生长。RT-PCR分析了两种不同的hap包被样品的Runx2、碱性磷酸酶、胶原蛋白和骨钙素。杆状纳米阵列涂层上的MC3T3-E1细胞具有较高的骨相关基因表达。这一发现提示HAP的有序组装结构可能引起与生物分子的地形依赖性协调，从而促进成骨样细胞的增殖和成骨分化。这项研究为生物材料的表面特征提供了一个了解，以确保更好的生物活性。
How the surface topography of hydroxyapatite (HAP) coatings remodels hard tissue is of utmost importance and a contributing factor to ambiguity regarding this subject. Here, HAP coatings with different topographies of a rod-like nanoarray with c-axis orientation and a flake-like micro-flower array with a(b)-axis orientation on a Ti substrate were synthesized via hydrothermal-electrodeposition by controlling the concentration of electrolytes. XRD, TEM and SAED analyses indicated that the rod-like HAP nanoarray was predominant with an orientation of (001), while the HAP micro-flower samples were based on an orientation of (100). Compared to the flake-like HAP, the rod-like nanoarray HAP possessed better hydrophilic properties and lower roughness, which not only enhanced adsorption of specific fibronectin proteins but also promoted the spreading and growth of MC3T3-E1 cells. Runx2, alkaline phosphatase, collagen and osteocalcin were also analyzed by RT-PCR on the two distinctive HAP-coated samples. MC3T3-E1 cells on the rod-like nanoarray coating had higher osteo-related gene expression. This finding suggested that the ordered assembly structure of the HAP might cause topographydependent coordination with biomolecules for enhancing osteoblast-like cell proliferation and osteogenic differentiation. This study provided an understanding of the surface's features for biomaterials to ensure better bioactivity.