Stable and specific expression of 4-coumarate:coenzyme A ligase gene (4CL1) driven by the xylem-specific Pto4CL1 promoter in the transgenic tobacco
Stable and specific expression of 4-coumarate:coenzyme A ligase gene (4CL1) driven by the xylem-specific Pto4CL1 promoter in the transgenic tobacco
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DOI：
10.1023/b:bile.0000035487.91628.9e
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Jiang, XN
中科院分区：
文献类型：
--
作者：
Lu, H;Zhao, YL;Jiang, XN
使用农杆菌介导的转化产生的转基因植物，研究了毛白杨 (Pto4CL1p) 的 4-香豆酸：辅酶 A 连接酶启动子驱动烟草中 GUS 报告基因和 4-香豆酸：辅酶 A 连接酶基因表达的能力。在携带 1140 bp Pto4CL1p 启动子的转基因烟草植物的茎木质部中检测到强烈的 GUS 组织化学染色。为了进一步研究组织特异性表达启动子Pto4CL1p的调控功能，将含有与4CL1基因融合的Pto4CL1p启动子的双元载体转入烟草中。 4CL1酶的活性在转基因烟草的茎中加倍，但在叶子中没有增加。转基因烟草茎中木质素含量增加了25%，但叶片中木质素含量没有增加。
The ability of 4-coumarate: coenzyme A ligase promoter from Populus tomentosa (Pto4CL1p) to drive expression of the GUS reporter gene and 4-coumarate: coenzyme A ligase gene in tobacco has been studied using transgenic plants produced by Agrobacterium-mediated transformation. Intense GUS histochemical staining was detected in the xylem of stem in transgenic tobacco plants carrying the 1140 bp Pto4CL1p promoter. To further investigate the regulation function of the tissue-specific expression promoter, Pto4CL1p, a binary vector containing Pto4CL1p promoter fused with 4CL1 gene was transferred into tobacco. The activity of the 4CL1 enzyme doubled in the stems of transgenic tobacco but did not increase in the leaves. The content of lignin was increased 25% in the stem but there was no increase in the leaves of transgenic tobacco.