Comparison of thick films, in vitro culture and DNA hybridization probes for detecting Plasmodium falciparum malaria.
Comparison of thick films, in vitro culture and DNA hybridization probes for detecting Plasmodium falciparum malaria.
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厚膜、体外培养和 DNA 杂交探针检测恶性疟原虫疟疾的比较。
DOI：
10.4269/ajtmh.1989.40.3
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Chulay
中科院分区：
文献类型：
--
作者：
D. Lanar;G. Mclaughlin;D. Wirth;R. Barker;J. Zolg;J. Chulay
使用患有子孢子诱发疟疾的志愿者的血液，对吉姆萨染色厚膜检查、体外培养和用于检测寄生虫血症的 4 种不同 DNA 探针的敏感性和特异性进行了比较。接种子孢子后 9 至 13 天，通过对 7 名志愿者的 0.5 微升血液进行厚膜检查，检测到明显的寄生虫血症（4-550 个寄生虫/微升）。子孢子接种后 7 天获得的 1 ml 血液培养物在最终出现明显寄生虫血症的所有志愿者中均呈阳性。 DNA 杂交探针仅在 5-28% 的涂片或培养阳性样本中检测到寄生虫。
Using blood from volunteers with sporozoite induced malaria, a comparison was made of the sensitivity and specificity of Giemsa stained thick film examination, in vitro culture, and 4 different DNA probes for detecting parasitemia. Between 9 and 13 days after sporozoite inoculation, patent parasitemia (4-550 parasites/microliters) was detected by thick film examination of 0.5 microliters blood in 7 volunteers. Cultures of 1 ml blood obtained 7 days after sporozoite inoculation were positive in all volunteers who eventually developed patent parasitemia. The DNA hybridization probes detected parasites in only 5-28% of smear- or culture-positive samples.