Open tubular capillary electrochromatography migration behavior of enkephalins in etched chemically modified fused silica capillaries.
Open tubular capillary electrochromatography migration behavior of enkephalins in etched chemically modified fused silica capillaries.
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DOI：
10.1016/j.chroma.2006.04.090
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发表时间：
2006-09
期刊：
影响因子：
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通讯作者：
J. Pesek;M. Matyska;S. Velpula
中科院分区：
文献类型：
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作者：
J. Pesek;M. Matyska;S. Velpula
在第二种无机盐（CuCl 2、CrCl 3、NaNO 3或（NH 4）2CO 3）的存在下，用氟化氢铵蚀刻用于电泳分析的熔融二氧化硅毛细管。这些无机成分的存在下，在表面基质上的脑啡肽的电迁移行为的影响进行了评估。分离度、效率和峰形用于比较各种色谱柱。在一些情况下，然后通过使用硅烷化/硅氢化过程添加十八烷基部分来改性蚀刻的表面。蚀刻毛细管的表面性质也可以通过电渗流测量来评估。相同实验条件下迁移时间的RSD <1%。
Fused silica capillaries for use in electrophoretic analyses are etched with ammonium bifluoride in the presence of a second inorganic salt (CuCl2, CrCl3, NaNO3, or (NH4)2CO3). The effects of the presence of these inorganic components in the surface matrix on the electromigration behavior of enkephalins are evaluated. Resolution, efficiency and peak shape are used to compare the various columns. In some cases the etched surface is then modified by the addition of an octadecyl moiety using a silanization/hydrosilation procedure. The surface properties of the etched capillaries can also be evaluated by electroosmotic flow measurements. RSDs of migration times under identical experimental conditions were <1%.