Neuroinflammation Quantification for Spinal Cord Injury.
Neuroinflammation Quantification for Spinal Cord Injury.
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DOI：
10.1002/cpim.57
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发表时间：
2018-11-01
影响因子：
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通讯作者：
Lopez-Vales, Ruben
中科院分区：
文献类型：
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作者：
Amo-Aparicio, Jesus;Martinez-Muriana, Anna;Lopez-Vales, Ruben
脊髓损伤（SCI）导致不可逆的破坏性神经功能障碍。文献中积累的证据表明，损伤后脊髓中发生的炎症反应对将组织损伤扩散到邻近病变部位的健康区域具有重要作用，因此增加了神经功能缺损。因此，靶向炎症可能导致新疗法的开发，以防止SCI后的组织损伤和神经功能障碍。炎症部分地通过主要由神经胶质细胞合成的促炎和抗炎细胞因子的表达来调节。因此，需要能够简化脊髓组织样品中多种细胞因子和免疫细胞的定量的方法来评估新抗炎疗法的潜力。在本单元中，我们描述了如何在小鼠脊髓中诱导挫伤，以及两种有用的方法来评估受损脊髓组织样本中的神经炎症。John Wiley & Sons，Inc.
Spinal cord injury (SCI) leads to irreversible devastating neurological disabilities. Accumulated evidence in the literature indicates that the inflammatory response that occurs in the spinal cord following injury contributes importantly to spread tissue damage to healthy regions adjacent to the lesion site, and consequently, to increase neurological deficits. Therefore, targeting inflammation could lead to the development of new therapies to prevent tissue damage and neurological impairments after SCI. Inflammation is regulated, in part, by the expression of pro-inflammatory and anti-inflammatory cytokines synthesized, mainly, by glial cells. Hence, methodologies that could ease the quantification of multiple cytokines and immune cells from spinal cord tissue samples are needed to assess the potential of new anti-inflammatory therapies. In the present unit, we describe how to induce contusion injuries in the mouse spinal cord, as well as, two useful methodologies to assess neuroinflammation in lesioned spinal cord tissue samples. © 2018 by John Wiley & Sons, Inc.