Positive ion fast atom bombardment mass spectrometry of reductively pyridylaminated maltooligosaccharides and its application to alpha-amylase assay.
Positive ion fast atom bombardment mass spectrometry of reductively pyridylaminated maltooligosaccharides and its application to alpha-amylase assay.
复制标题
还原吡啶胺化麦芽低聚糖的正离子快原子轰击质谱及其在 α-淀粉酶测定中的应用。
DOI：
10.1002/bms.1200150408
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Morita
中科院分区：
文献类型：
--
作者：
S. Honda;K. Kakehi;T. Ohmura;M. Morita
用2-氨基吡啶还原的麦芽寡糖(Gn-AP)的正离子快原子轰击质谱图中含有丰富的[M+H]+离子。通过α-淀粉酶的作用测定G5-AP产生的G2-AP和G3-AP，根据它们的[M+H]+离子相对于以2-氨基-6-甲基吡啶为内标还原氨化的纤维二糖的丰度，可以快速和重复性地测定这些酶。测定胰腺和唾液中α-淀粉酶活性的比例有利于临床研究。
Positive ion fast atom bombardment mass spectra of maltooligosaccharides reductively aminated with 2-aminopyridine (Gn-AP) contain abundant [M + H]+ ions. Determination of G2-AP and G3-AP produced from G5-AP by the action of alpha-amylases, based on the abundance of their [M + H]+ ions relative to that of cellobiose reductively aminated with 2-amino-6-methylpyridine as the internal standard, allowed rapid and reproducible assay of these enzymes. It was advantageous for clinical investigation that the proportion of pancreatic and salivary alpha-amylase activities could be determined.