Repair kinetics of gamma-ray induced DNA damage determined by the single cell gel electrophoresis assay in murine leukocytes in vivo
Repair kinetics of gamma-ray induced DNA damage determined by the single cell gel electrophoresis assay in murine leukocytes in vivo
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DOI：
10.1016/s0921-8777(98)00061-5
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发表时间：
1999-01-26
期刊：
影响因子：
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通讯作者：
Morales-Ramírez, P
中科院分区：
文献类型：
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作者：
Mendiola-Cruz, MT;Morales-Ramírez, P
用彗星法测定了γ射线诱导的小鼠外周血白细胞体内DNA损伤的修复动力学。将小鼠暴露于（137）Cs源中1.0 Gy的伽马射线中，并在不同时间从其尾部采集外周血样本。在单独的实验中，通过测量每个样本中带尾（彗星）细胞的比例来评估每只小鼠的修复情况。平均近80%的彗星是在曝光后的最初时间获得的；2分钟后，频率下降到45%，15分钟时继续下降到22%。这证明了快速修复机制的存在。在暴露后的25到40分钟内，彗星的数量从22%增加到38%，随后又出现了第二次减少，这可能是由于后期修复过程导致链断裂，然后再连接它们。总之，我们的结果表明，该系统似乎适合于研究电离辐射暴露后细胞的修复能力。(C) 1999 Elsevier Science B.V.版权所有
The repair kinetics of the gamma rays induced DNA damage was determined in murine peripheral blood leukocytes in vivo by the comet assay. Mice were exposed to 1.0 Gy of gamma rays in a (137)Cs source and samples of peripheral blood were taken from their tails at different times. The repair was evaluated per mice in separate experiments by measuring the proportion of cells with tail (comets) in each sample. An average of nearly 80% of comets was obtained at the initial time after the exposure; 2 min later the frequency decreased to 45% and continued diminishing to 22% at 15 min. This evidences the presence of a rapid repair mechanism. For a period of 25 to 40 min after exposure there was a slight but consistent increase of comets from 22 to 38% followed by a second reduction, which could be due to a late repair process that causes strand breaks and then joined them. In summary our results indicated that this system seems to be appropriate for the study of the repair capacity of cells following exposure to ionizing radiation. (C) 1999 Elsevier Science B.V. All rights reserved.