Natural microbial communities supporting the transfer of the IncP-1β plasmid pB10 exhibit a higher initial content of plasmids from the same incompatibility group
Natural microbial communities supporting the transfer of the IncP-1β plasmid pB10 exhibit a higher initial content of plasmids from the same incompatibility group
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DOI：
10.3389/fmicb.2014.00637
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发表时间：
2014-11-24
影响因子：
5.2
通讯作者：
Merlin, Christophe
中科院分区：
文献类型：
--
作者：
Bellanger, Xavier;Guilloteau, Helene;Merlin, Christophe
质粒介导的抗生素耐药基因转移是公共卫生关注的问题，但支持质粒传播的容许环境仍然很难识别。最近，我们报道了一种基于定量PCR（qPCR）的分子方法来监测IncP-1 β质粒pB 10在微宇宙中维持的天然微生物群落中的命运。用13种不同的环境基质进行了这样的质粒转移实验，并证明了在复杂环境中接合活性质粒pB 10的转移是相对罕见的，并且是强烈的基质依赖性的。试图将微生物群落结构和基质容许性联系起来，结果表明TTGE分析的分辨率不足以指出支持pB 10转移的可比群落之间的共同特征。然而，通过qPCR对IncP-1 α/IncP-1 β质粒丰度的估计表明，pB 10转移倾向于得到表现出较高IncP-1质粒含量的环境基质的支持。我们认为，在一个给定的微生物群落中的IncP-1质粒的相对丰度反映了它的允许转让属于同一不相容组，这战胜了转让限制由于被称为超感染免疫的现象的质粒。
Antibiotic resistance gene transfer mediated by plasmids is a matter of concern for public health, but permissive environments supporting plasmid dissemination are still quite difficult to identify. Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1 beta plasmid pB10 in natural microbial communities maintained in microcosms. Such plasmid transfer experiments were carried out with 13 different environmental matrices, and demonstrated that the transfer of the conjugative-proficient plasmid pB10 in complex environments is relatively rare and is strongly matrix dependent. An attempt to link the microbial community structure and the matrix permissiveness showed that TTGE analysis is not resolutive enough to point out common features among comparable communities supporting pB10 transfer. However, an estimation of the IncP-1 alpha/IncP-1 beta plasmids abundance by qPCR demonstrated that pB10 transfer tends to be supported by environmental matrices exhibiting a higher content of IncP-1 plasmids. We suggest that the relative abundance of IncP-1 plasmids in a given microbial community reflects its permissiveness to the transfer of plasmids belonging to the same incompatibility group, which prevails over transfer limitation due to a phenomenon known as superinfection immunity.