Mapping lumbar efferent and afferent spinal circuitries via paddle array in a porcine model.
Mapping lumbar efferent and afferent spinal circuitries via paddle array in a porcine model.
复制标题
通过猪模型中的桨阵列绘制腰椎传出和传入脊髓回路。
DOI：
10.1016/j.jneumeth.2024.110104
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发表时间：
2024
影响因子：
3
通讯作者：
Sayenko,DG
中科院分区：
文献类型：
--
作者：
Steele,AG;Taccola,G;Frazier,AM;Manzella,M;Hogan,M;Horner,PJ;Faraji,AH;Sayenko,DG
背景临床前模型对于识别神经损伤后发生的变化和评估治疗干预是必不可少的。尤卡坦小型猪（minipigs）的大脑和脊髓尺寸与人类相似，可用于实验室到临床研究。然而，很少有工作已经做了映射脊髓sensorimotor分布和识别猪和人类spinal cords.New methodsTo表征传出和传入信号之间的相似性和差异，我们植入了一个传统的32接触，四列阵列到背侧硬膜外腔腰骶脊髓，跨越L5-L 6椎骨，在两个尤卡坦小型猪。脊髓诱发运动电位记录双侧在四个后肢肌肉刺激期间从不同的阵列位置。然后，脊髓背电位记录通过阵列刺激左，右胫nerves. ResultsUsing硬膜外脊髓刺激，我们实现了选择性的左，右，近端和远端激活在后肢肌肉。然后，我们确定了每一块肌肉的选择性作为一个函数的刺激位置，涉及到的分布的腰椎motor pools.Comparison与现有的methodsMapping运动神经元分布到后肢肌肉和记录反应，在背侧硬膜外间隙周围神经刺激揭示洞察上升和下降的信号传播在腰髓。临床级阵列尚未被利用在猪model.ConclusionsThese结果表明，传出和传入脊髓感觉运动网络是空间上不同的，提供有关组织的运动池在腰椎扩大的信息，并证明在大型动物研究中使用临床级设备的可行性。
BackgroundPreclinical models are essential for identifying changes occurring after neurologic injury and assessing therapeutic interventions. Yucatan miniature pigs (minipigs) have brain and spinal cord dimensions like humans and are useful for laboratory-to-clinic studies. Yet, little work has been done to map spinal sensorimotor distributions and identify similarities and differences between the porcine and human spinal cords.New methodsTo characterize efferent and afferent signaling, we implanted a conventional 32-contact, four-column array into the dorsal epidural space over the lumbosacral spinal cord, spanning the L5–L6 vertebrae, in two Yucatan minipigs. Spinally evoked motor potentials were recorded bilaterally in four hindlimb muscles during stimulation delivered from different array locations. Then, cord dorsum potentials were recorded via the array by stimulating the left and right tibial nerves.ResultsUtilizing epidural spinal stimulation, we achieved selective left, right, proximal, and distal activation in the hindlimb muscles. We then determined the selectivity of each muscle as a function of stimulation location which relates to the distribution of the lumbar motor pools.Comparison with existing methodsMapping motoneuron distribution to hindlimb muscles and recording responses to peripheral nerve stimulation in the dorsal epidural space reveals insights into ascending and descending signal propagation in the lumbar spinal cord. Clinical-grade arrays have not been utilized in a porcine model.ConclusionsThese results indicate that efferent and afferent spinal sensorimotor networks are spatially distinct, provide information about the organization of motor pools in the lumbar enlargement, and demonstrate the feasibility of using clinical-grade devices in large animal research.