Escherichia coli with extended-spectrum beta-lactamases or transferable AmpC beta-lactamases and Salmonella on meat imported into Sweden
Escherichia coli with extended-spectrum beta-lactamases or transferable AmpC beta-lactamases and Salmonella on meat imported into Sweden
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DOI：
10.1016/j.ijfoodmicro.2013.11.005
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发表时间：
2014-02-03
影响因子：
5.4
通讯作者：
Lindblad, Mats
中科院分区：
文献类型：
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作者：
Egervarn, Maria;Borjesson, Stefan;Lindblad, Mats
越来越多的报道称，全世界人类和动物体内存在产生超广谱 β-内酰胺酶 (ESBL) 或可转移 AmpC β-内酰胺酶 (pAmpC) 的肠杆菌科细菌。它们在食用动物中的出现表明肉类可能是这两个种群之间的联系。本研究调查了瑞典进口的 430 个牛肉、猪肉和鸡肉样品中沙门氏菌和产 ESBL 或 pAmpC 的大肠杆菌的发生情况和特征，以便为评估食品中这些细菌的潜在公共卫生风险提供所需的数据。根据原产地的不同，0-8% 的牛肉样品、2-13% 的猪肉样品和 15-95% 的肉鸡肉样品中发现了产 ESBL/pAmpC 的大肠杆菌。患病率最高的是南美肉鸡肉（95%），其次是来自欧洲（不包括丹麦）的肉鸡肉（61%）和丹麦肉鸡肉（15%）。来自斯堪的纳维亚半岛以外肉类的分离株通常被定义为具有多重耐药性。大多数 ESBL/pAmpC 基因可通过接合转移。 Bla(CTX-M-2) 和 bla(CTX-M-8) 是南美肉鸡肉中大肠杆菌的显性基因，而 bla(CMY-2) 和 bla(CTX-M-1) 在欧洲肉中占主导地位。 bla(CMY-2) 和 bla(CTX-M-1) 的大部分分别位于复制子类型 incK 和 incI1 的质粒上。 ESBL/pAmpC 基因和质粒的相同组合先前已在临床人类分离株中描述过。在来自欧洲猪肉和肉鸡肉的五个测试样品中发现了沙门氏菌。没有分离出的沙门氏菌对第三代头孢菌素耐药。总之，进口到瑞典的肉类，特别是肉鸡肉，是人类接触产 ESBL 和 pAmpC 大肠杆菌的潜在来源。 (C) 2013 Elsevier B.V. 保留所有权利。
The presence of Enterobacteriaceae producing extended spectrum beta-lactamases (ESBL) or transferable AmpC beta-lactamases (pAmpC) is increasingly being reported in humans and animals world-wide. Their occurrence in food-producing animals suggests that meat is a possible link between the two populations. This study investigated the occurrence and characteristics of Salmonella and ESBL- or pAmpC-producing E. coli in 430 samples of beef, pork and broiler meat imported into Sweden, in order to provide data required for assessing the potential public health risk of these bacteria in food. Depending on region of origin, ESBL/pAmpC-producing E. coli were found in 0-8% of beef samples, 2-13% of pork samples and 15-95% of broiler meat samples. The highest prevalence was in South American broiler meat (95%), followed by broiler meat from Europe (excluding Denmark) (61%) and from Denmark (15%). Isolates from meat outside Scandinavia were generally defined as multiresistant A majority of the ESBL/pAmpC genes were transferable by conjugation. Bla(CTX-M-2) and bla(CTX-M-8) were the dominant genes in E. coli from South American broiler meat, whereas bla(CMY-2) and bla(CTX-M-1) dominated in European meat. The majority of bla(CMY-2) and bla(CTX-M-1) were situated on plasmids of replicon type incK and incI1, respectively. The same combinations of ESBL/pAmpC genes and plasmids have been described previously in clinical human isolates. Salmonella was found in five samples tested, from European pork and broiler meat. No Salmonella isolate was resistant to third-generation cephalosporins. In conclusion, meat imported into Sweden, broiler meat in particular, is a potential source of human exposure to ESBL- and pAmpC-producing E. coli. (C) 2013 Elsevier B.V. All rights reserved.