Separation of brain monosialoganglioside molecular species by high-performance liquid chromatography.
Separation of brain monosialoganglioside molecular species by high-performance liquid chromatography.
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DOI：
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发表时间：
1984-10
影响因子：
6.5
通讯作者：
H. Kadowaki;J. Evans;R. McCluer
中科院分区：
文献类型：
--
作者：
H. Kadowaki;J. Evans;R. McCluer
本文介绍了一种用高效液相色谱法分离脑单唾液酸神经节苷脂分子种类的方法。从人脑中纯化GM 4、GM 3、GM 2和GM 1，并在C18反相柱上分离它们的各个分子种类。通过完整神经节苷脂的质谱法、脂肪酸的气液色谱法和长链碱基的高效液相色谱法鉴定峰。分子种类的特征洗脱序列允许基于其在反相柱上的保留时间对其进行鉴定。
A method for the separation of molecular species of brain monosialogangliosides by high-performance liquid chromatography is described. GM4, GM3, GM2, and GM1 were purified from human brain and their individual molecular species were separated on a C18 reversed-phase column. Peaks were identified by mass spectrometry of the intact ganglioside, by gas-liquid chromatography of the fatty acids, and by high-performance liquid chromatography of the long chain bases. A characteristic elution sequence of molecular species permitted their identification based upon their retention times on the reversed-phase column.