Phytic acid functionalized magnetic bimetallic metal-organic frameworks for phosphopeptide enrichment.
Phytic acid functionalized magnetic bimetallic metal-organic frameworks for phosphopeptide enrichment.
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DOI：
10.1039/d0tb02517h
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发表时间：
2021-01
期刊：
影响因子：
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通讯作者：
Shuang Yan;Bin Luo;Jia He;Fang Lan;Yao Wu
中科院分区：
文献类型：
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作者：
Shuang Yan;Bin Luo;Jia He;Fang Lan;Yao Wu
由于磷酸肽在复杂生物样品中的重要性和微量性，因此，对磷酸肽的高特异性富集是深入研究其生理和病理过程的前提。本工作采用一种简单有效的方法制备了植酸（PA）功能化的磁性铈锆复合金属有机骨架纳米复合材料（Fe 3 O 4 @SiO2@Ce-Zr-MOF@PA）。该纳米材料具有较高的灵敏度（0.1 fmol μL-1），对β-酪蛋白胰蛋白酶/牛血清白蛋白（1：800）磷酸肽具有较高的选择性，并具有良好的重复使用性。将此亲和探针应用于生物样品，从脱脂牛奶、人血清和人唾液中分别鉴定出19、4和15个磷酸肽。上述显著的优点归因于在MOF壳的表面上的丰富的Ce-O和Zr-O纳米团簇的强亲和力以及来自大量磷酸基团的改善的亲水性。因此，新型Fe 3 O 4 @SiO2@Ce-Zr-MOF@PA纳米微球不仅能有效富集磷酸肽，还能降低磷酸肽的吸附，在复杂生物样品中低丰度磷酸肽的鉴定和进一步分析中具有很大的潜力。
Highly specific enrichment of phosphopeptides from complex biological samples was a precondition for further studying its physiological and pathological processes due to the important and trace amounts of phosphopeptides. In this work, phytic acid (PA) functionalized magnetic cerium and zirconium bimetallic metal-organic framework nanocomposites (denoted as Fe3O4@SiO2@Ce-Zr-MOF@PA) were fabricated by a facile yet efficient method. The as-prepared nanomaterial exhibited high sensitivity (0.1 fmol μL-1), high selectivity toward phosphopeptides from β-casein tryptic digests/BSA (1 : 800), and good reusability of five cycles for enriching phosphopeptides. This affinity probe was applied to biological samples, and 19, 4 and 15 phosphopeptides were identified from non-fat milk, human serum and human saliva, respectively. The above marked advantages are attributed to the strong affinity of the abundant Ce-O and Zr-O nanoclusters on the surface of the MOF shell with the improved hydrophilicity from a great number of phosphate groups. Therefore, the novel Fe3O4@SiO2@Ce-Zr-MOF@PA nanospheres could not only enrich phosphopeptides effectively, but also reduce the adsorption of phosphopeptides, manifesting great potential in the identification and further analysis of low abundance phosphopeptides in complex biological samples.