Fluorescence polarization of 1,6‐diphenyl‐1,3,5‐hexatriene embedded in membranes of mouse leukemic L 1210 cells during the cell cycle
Fluorescence polarization of 1,6‐diphenyl‐1,3,5‐hexatriene embedded in membranes of mouse leukemic L 1210 cells during the cell cycle
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细胞周期期间嵌入小鼠白血病 L 1210 细胞膜中的 1,6-二苯基-1,3,5-己三烯的荧光偏振
DOI：
10.1016/0014-5793(78)80170-7
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发表时间：
1978
期刊：
影响因子：
3.5
通讯作者：
M. Monsigny
中科院分区：
文献类型：
--
作者：
A. Obrénovitch;C. Sené;M. Negre;M. Monsigny
在细胞周期中发生的许多事件中，有一些发生在细胞表面膜水平上。通过用荧光凝集素标记正常细胞和转化细胞，证实了细胞周期中发生的变化[1-3]。在正常细胞中，凝集素的结合在有丝分裂期间增加，在转化细胞中，凝集素的结合在间期增加。此外，膜内颗粒在细胞周期的几个阶段聚集，但在有丝分裂阶段没有聚集。最近，有研究表明，当细胞密度增加时，正常和转化的哺乳动物成纤维细胞的流动性降低[5,6]，而当细胞受到刺激生长时，各种哺乳动物细胞的流动性增加。这些结果表明，细胞膜在细胞周期的不同阶段发生了动态变化。在本报告中，小鼠白血病细胞l1210与colcolid同步，并通过嵌入脂质双分子层的1,6 -二苯基- 1,3,5 -六三烯[8]的荧光偏振测量来检查预期的变化。这些结果是在非同步l1210细胞[21]中掺入DPH的荧光衰减研究的基础上讨论的。
Amongst the numerous events which occur during a cell cycle, several are localized at the level of cell surface membrane. By labelling normal and transformed cells with fluorescent lectins, changes occurring during the cell cycle have been demonstrated [1-3]. With normal cells, the binding of lectins increased during mitosis and with transformed cells it increased during interphase. Furthermore, intramembranous particles were aggregated during several phases of the cell cycle but not during the mitotic phase [4]. More recently, it was suggested that the fluidity of normal and transformed mammalian flbroblasts in culture decreased when the cell density increased [5, 6] and that the fluidity of various mammalian cells increased [7] when cells were stimulated for growth. All these results suggest that the cell membrane undergoes a dynamic change during different phases of cell cycle. In the present report, murine leukemic cells L 1210 were synchronized with colcemid and the expected changes were checked by fluorescence polarization measurements of 1, 6-diphenyl-l, 3, 5-hexatriene [8] embedded in lipid bilayer. These results are discussed on the basis of fluorescence decay studies of DPH incorporated in unsynchronized L 1210 cells [21].