Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes
Quercetin protects against ethanol-induced oxidative damage in rat primary hepatocytes
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DOI：
10.1016/j.tiv.2009.03.006
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发表时间：
2010-03-01
影响因子：
3.2
通讯作者：
Liu, Liegang
中科院分区：
文献类型：
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作者：
Liu, Shuang;Hou, Wei;Liu, Liegang
酒精性肝病(AID)的发生和发展与自由基损伤和氧化应激有关，抗氧化剂和自由基清除剂可部分减轻AID的作用。栎素是植物中分布最广泛的黄酮类化合物之一，是一种天然抗氧化剂。探讨了槲皮素对乙醇所致肝细胞氧化损伤的保护作用。乙醇染毒(100 mM×8h)大鼠原代肝细胞，分别用10 0 mM乙醇染毒后处理(2 H)、同时处理(2 H)和预先处理(2 H)，采用时间依赖性(0.5~8h)和剂量依赖性(2 5~2 0 0 mM)的方法。通过测定乳酸脱氢酶(LDH)、天冬氨酸氨基转移酶(AST)、丙二醛(MDA)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)等指标的变化，探讨槲皮素干预后细胞损伤和抗氧化状态的变化。在乙醇染毒前2~4h，用50 mU M的槲皮素预先处理肝细胞2~4h后，上述指标基本恢复到对照组水平。结论：槲皮素预处理对乙醇诱导的肝细胞氧化应激具有保护作用，可作为预防和/或治疗酒精性肝病的一种新型天然药物。(C)2009爱思唯尔有限公司。保留所有权利。
The pathogenesis and progression of alcoholic liver disease (AID) are associated with free radical injury and oxidative stress, which could be partially attenuated by antioxidants and free radical scavengers. Quercetin, one of the most widely distributed flavonoids in plants, is a natural antioxidant. The hypothesis that quercetin could prevent the ethanol-induced oxidative damage in hepatocytes was investigated. The ethanol-intoxicated (100 mM for 8 h) rat primary hepatocytes were post-treated (2 h), simultaneously treated or pre-treated (2 h) with quercetin respectively, while the time-dependent (0.5-8 h) and dose-dependent (25-200 mu M) quercetin pre-treatment were used in the present study. The parameters of lactate dehydrogenase (LDH), aspartate transaminase (AST), malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were determined to address the alterations of cell damage and antioxidant state after quercetin intervention. The toxic insult of ethanol to hepatocytes was challenged by quercetin and these parameters almost returned to the level of control group when hepatocytes were pre-treated with quercetin at the dose of 50 mu M for 2-4 h before ethanol exposure. In conclusion, quercetin pre-treatment provided protection against ethanol-induced oxidative stress in hepatocytes and may be used as a new natural drug for the prevention and/or treatment of ALD. (C) 2009 Elsevier Ltd. All rights reserved.