A PCR-based survey of homeobox genes in Ctenodrilus serratus (Annelida: Polychaeta).
A PCR-based survey of homeobox genes in Ctenodrilus serratus (Annelida: Polychaeta).
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基于 PCR 的锯线藻同源盒基因调查（环节动物门：多毛类）。
DOI：
10.1006/mpev.1994.1017
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发表时间：
1994
影响因子：
4.1
通讯作者：
Buss,LW
中科院分区：
文献类型：
--
作者：
Dick,MH;Buss,LW
受访的多毛纲的wormCtenodrilus serratuswas轨的存在/ HOX和engrailed-type同源框基因用简并引物PCR。用三种不同的引物对基因组和cDNA进行调查，发现16个独特的同源盒片段。对于三个片段，RACE获得了额外的同型盒序列和3 '侧翼区域。鉴定出9个hox / hox型片段，包括Hox1/lab、Hox2/pb、Hox3、Hox4/Dfd和Antp/Lox5同源群的推定代表。另外两个antp样片段不能被指定特定的同源性。除了aUbx/ abda样基因外，还存在leechlox2的同源基因，这表明在环节动物和节肢动物谱系中存在单个前体的独立复制。没有确定Hox9/AbdB组的代表。我们的结果与单一HOX /HOX集群的假设是一致的，就像在强烈标记化的节肢动物中看到的那样，这表明这些基因的原始作用，即使在明显的异聚动物中，也不是明显的节段分化的具体说明。所使用的引物还检测到其他六个同源盒类或家族的代表：Xlox (XlHbox8/HTr-A2), Ovx (Chox7), caudal, Prh（富含脯氨酸的同源盒），NEC （ceh-9/Tghbox5）和engrailed。
The polychaete wormCtenodrilus serratuswas surveyed for the presence of HOM/HOX and engrailed-type homeobox genes using PCR with degenerate primers. Sixteen unique homeobox fragments were found in surveys of genomic and cDNA with three different primer sets. For three fragments, RACE was employed to obtain additional homeobox sequence and the 3′ flanking region. Nine HOM/HOX-type fragments were identified, including putative representatives of the Hox1/lab, Hox2/pb, Hox3, Hox4/Dfd, and Antp/Lox5 cognate groups. Two additionalAntp-like fragments could not be assigned specific orthology. Presence of an ortholog of leechLox2in addition to aUbx/abdA-like gene suggests that independent duplications of a single precursor occurred in the annelid and arthropod lineages. No representative of the Hox9/AbdB group was identified. Our results are consistent with a hypothesis of a single HOM/HOX cluster inCtenodrilusas extensive as that seen in strongly tagmatized arthropods, suggesting that the primitive role of these genes even in overtly metameric animals was something other than specification of overt segmental differentiation. The primers used also detected representatives of six other homeobox classes or families: Xlox (XlHbox8/HTr-A2), Ovx (Chox7), caudal, Prh (proline-rich homeobox), NEC (ceh-9/Tghbox5), and engrailed.