Drying of open animal joints in vivo subsequently causes cartilage degeneration.
Drying of open animal joints in vivo subsequently causes cartilage degeneration.
复制标题
在体内开放动物关节的干燥随后会导致软骨变性。
DOI：
10.1302/2046-3758.54.2000594
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发表时间：
2016-04
影响因子：
4.6
通讯作者：
Hall AC
中科院分区：
文献类型：
--
作者：
Paterson SI;Eltawil NM;Simpson AH;Amin AK;Hall AC
在开放性骨科手术中，关节可能暴露于空气中，可能导致软骨干燥和软骨细胞死亡，然而，关节干燥在体内的长期影响知之甚少。我们使用动物模型来研究关节干燥对软骨和软骨细胞的后续影响。将麻醉大鼠的髌骨沟暴露（假手术），或暴露然后经受层流气流（0.25m/s; 60分钟），然后缝合伤口并使动物恢复。监测动物长达8周，然后处死。软骨和软骨细胞的性质进行了研究，分别通过组织学和共聚焦显微镜。关节干燥导致软骨表面区域内广泛的软骨细胞死亡。干燥软骨的组织学显示在4周时表面完整性丧失，在8周时纤维化，并且改良的Mankin评分增加（p < 0.001）。软骨厚度增加（p < 0.001），而软骨细胞密度在四周时降低（p < 0.001），但随后在八周时增加至假手术水平（p < 0.01）。到第8周，软骨细胞配对/聚集和细胞体积增加（分别为p < 0.05; p < 0.001）。这些体内结果首次证明，由于层流气流，发生软骨退化，其特征与早期骨关节炎中所见相似。因此，在开放性骨科手术中保持足够的软骨水合作用至关重要。本文作者：Dr A.大厅体内开放的动物关节的干燥随后引起软骨退化。骨关节研究2016;5：137-144。DOI：10.1302/2046-3758.54.2000594。
During open orthopaedic surgery, joints may be exposed to air, potentially leading to cartilage drying and chondrocyte death, however, the long-term effects of joint drying in vivo are poorly understood. We used an animal model to investigate the subsequent effects of joint drying on cartilage and chondrocytes. The patellar groove of anaesthetised rats was exposed (sham-operated), or exposed and then subjected to laminar airflow (0.25m/s; 60 minutes) before wounds were sutured and animals recovered. Animals were monitored for up to eight weeks and then sacrificed. Cartilage and chondrocyte properties were studied by histology and confocal microscopy, respectively. Joint drying caused extensive chondrocyte death within the superficial regions of cartilage. Histology of dried cartilage demonstrated a loss of surface integrity at four weeks, fibrillations at eight weeks, and an increased modified Mankin score (p < 0.001). Cartilage thickness increased (p < 0.001), whereas chondrocyte density decreased at four weeks (p < 0.001), but then increased towards sham-operated levels (p < 0.01) at eight weeks. By week eight, chondrocyte pairing/clustering and cell volume increased (p < 0.05; p < 0.001, respectively). These in vivo results demonstrated for the first time that as a result of laminar airflow, cartilage degeneration occurred which has characteristics similar to those seen in early osteoarthritis. Maintenance of adequate cartilage hydration during open orthopaedic surgery is therefore of paramount importance. Cite this article: Dr A. Hall. Drying of open animal joints in vivo subsequently causes cartilage degeneration. Bone Joint Res 2016;5:137–144. DOI: 10.1302/2046-3758.54.2000594.