Responsive hybrid hydrogels with volume transitions modulated by a titin immunoglobulin module
Responsive hybrid hydrogels with volume transitions modulated by a titin immunoglobulin module
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DOI：
10.1021/bc000046h
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发表时间：
2000-09-01
影响因子：
4.7
通讯作者：
Stewart, RJ
中科院分区：
文献类型：
--
作者：
Chen, L;Kopecek, J;Stewart, RJ
利用人心肌肌动蛋白的I28类免疫球蛋白(Ig)模块，将丙烯酰胺(AAM)共聚物交联成杂化水凝胶。交联是通过I28模块的末端组氨酸标签(His标签)与共聚物上含有金属螯合的氮三乙酸(NTA)侧链之间的金属配位键合实现的。在溶液中，I28模块的Beta-Sheet结构随着温度的升高而展开，转变中点约为58摄氏度。与I28模块交联的水凝胶表现出正的温度响应性；当温度高于交联物的熔融温度时，它们膨胀到其初始体积的3倍。对于合成水凝胶来说，正温度响应性是不寻常的。I28杂化水凝胶表明，将合成聚合物与天然的、特性良好的蛋白质模块进行交联是创造具有独特环境响应性的新材料的实用策略，这种独特的环境响应性由蛋白质交联物的机械性能决定。这些新材料可能对受控化学输送有用。
The I28 immunoglobulin (Ig)-like module of human cardiac titin, an elastic muscle protein, was used to cross-link acrylamide (AAm) copolymers into hybrid hydrogels. Cross-linking was accomplished through metal coordination bonding between terminal histidine tags (His tags) of the I28 module and metal-chelating nitrilotriacetic acid (NTA)-containing side chains on the copolymer. In solution, the beta-sheet structure of the I28 module unfolded with a transition midpoint of about 58 degrees C as the temperature was elevated. Hydrogels cross-linked with the I28 module demonstrated positive temperature responsiveness; they swelled to 3 times their initial volume at temperatures above the melting temperature of the cross-links. Positive temperature responsiveness is unusual for synthetic hydrogels. The I28 hybrid hydrogels demonstrate that cross-linking synthetic polymers with natural, well-characterized protein modules is a practical strategy for creating new materials with unique environmental responsiveness predictably determined by the mechanical properties of the protein cross-links. These new materials may be useful for controlled chemical delivery.