Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA
Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA
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DOI：
10.1360/02tb9184
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发表时间：
2002-05-01
影响因子：
--
通讯作者：
Yue, M
中科院分区：
文献类型：
--
作者：
Han, R;Wang, XL;Yue, M
利用琼脂糖凝胶电泳法和环丁基嘧啶二聚体(CPD)限制性内切酶T4-内切脱氧核糖核酸酶-V，研究了5 mW(.)Mm(-2)照射对增强UV-B(10.08kJ(.)M(-2.)D(-1))辐射的影响。结果表明，He-Ne激光照射后，细胞内形成的核酸内切酶敏感部位(ESS)含量明显减少。在He-Ne激光照射下，小麦细胞内T4-内脱氧核糖核酸酶-V消化的内切酶敏感部位(ESS)CPD被切除，单链断裂(SSB)含量减少。
Using agarose gel electrophoresis and T4-endodeoxyribonuclease-V, which is a kind of restriction endonuclease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5 mW (.) mm(-2)) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08 kJ (.) m(-2 .) d(-1)) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-V had been stimulated in the wheat cells.