A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway
A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway
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DOI：
10.1038/ncb1751
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发表时间：
2008-08-01
影响因子：
21.3
通讯作者：
Luini, Alberto
中科院分区：
文献类型：
--
作者：
Pulvirenti, Teodoro;Giannotta, Monica;Luini, Alberto
与其他复杂的细胞功能一样，细胞膜内运输涉及一系列细胞器和机械的协调参与；然而，这种配位的分子基础是未知的。在这里，我们描述了一个基于高尔基的信号系统，该系统由交通激活，并参与监测和平衡进出高尔基复合体的贩运率。我们提供的证据表明，交通信号是由于蛋白质伴侣离开内质网到达高尔基复合体，在那里它们与KDEL受体结合。这引发了一个信号反应，包括激活Src激酶的高尔基池和磷酸化级联，进而激活高尔基体内部运输，从而维持高尔基体复合物的动态平衡。从这项研究中出现的概念应该有助于理解协调高阶细胞功能的控制电路。
As with other complex cellular functions, intracellular membrane transport involves the coordinated engagement of a series of organelles and machineries; however, the molecular basis of this coordination is unknown. Here we describe a Golgi-based signalling system that is activated by traffic and is involved in monitoring and balancing trafficking rates into and out of the Golgi complex. We provide evidence that the traffic signal is due to protein chaperones that leave the endoplasmic reticulum and reach the Golgi complex where they bind to the KDEL receptor. This initiates a signalling reaction that includes the activation of a Golgi pool of Src kinases and a phosphorylation cascade that in turn activates intra-Golgi trafficking, thereby maintaining the dynamic equilibrium of the Golgi complex. The concepts emerging from this study should help to understand the control circuits that coordinate high-order cellular functions.