Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance
Polymorphisms of myostatin gene (MSTN) in four goat breeds and their effects on Boer goat growth performance
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四个山羊品种肌肉生长抑制素基因(MSTN)多态性及其对波尔山羊生长性能的影响
DOI：
10.1007/s11033-011-1071-0
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发表时间：
2012-03-01
影响因子：
2.8
通讯作者：
Yang, Liguo
中科院分区：
文献类型：
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作者：
Zhang, Chunyan;Liu, Yun;Yang, Liguo
采用基因测序和聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对687名山羊个体的肌生长抑制素（Myostatin，MSTR）基因多态性进行了研究。在4个猪品种中检测到5 bp indel（1，256 TTTTA/-）的3种潜在基因型（AA、AB和BB）。第1外显子1, 388 T/A的CC、CD和DD多态性仅在波尔山羊中存在分离。与BB型相比，AB型猪初生重、90日龄体重、300日龄体重和初生体长显著增加（P < 0.05）。CD基因型的0号和90号体重、初生体高显著高于CC基因型（P < 0.05）。AB和CD基因型个体在其他体尺指标上也有较大差异，但差异不显著（P > 0.05）。组合时，组合基因型ABCD对BW 0、BW 90、BW 300、BH和BCG的生长性状有显著的正效应（P < 0.05）。这些结果表明肌生长抑制素（MSTN）中的这些标记与波尔山羊的生长相关，并且可能有助于标记辅助选择。
Polymorphisms of myostatin (MSTN) gene were investigated as a candidate marker for goat growth in 687 individuals by gene sequencing and polymerase chain reaction restriction fragment length polymorphism methods. Three potential genotypes (AA, AB and BB) of 5 bp indel (1,256 TTTTA/-) in 5'UTR were detected in four breeds. The polymorphism (CC, CD and DD) of substitution (1,388 T/A) in exon 1 was only segregating in Boer. Genotype AB resulted in significant increases in body weights at birth (BW0), 90 (BW90) and 300 (BW300) days of age, and birth body length compared to BB (P < 0.05). Whilst genotype CD contributed to heavier BW0 and BW90, and larger birth body height (BH) compared to CC (P < 0.05). Individuals either with AB or CD genotype also had greater values in other body sizes, although no significant differences appeared (P > 0.05). When in combination, the combined genotype ABCD displayed positive impacts on better growth traits in BW0, BW90, BW300, BH and BCG (P < 0.05). These results indicate that these markers in myostatin (MSTN) are associated with Boer growth and may be useful for marker assisted selection.