Anoxynatronum sibiricum gen.nov., sp.nov alkaliphilic saccharolytic anaerobe from cellulolytic community of Nizhnee Beloe (Transbaikal region)
Anoxynatronum sibiricum gen.nov., sp.nov alkaliphilic saccharolytic anaerobe from cellulolytic community of Nizhnee Beloe (Transbaikal region)
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DOI：
10.1007/s00792-002-0312-5
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发表时间：
2003-06-01
期刊：
影响因子：
2.9
通讯作者：
Lysenko, AM
中科院分区：
文献类型：
--
作者：
Garnova, ES;Zhilina, TN;Lysenko, AM
从贝加湖Nizhnee Beloye的纤维素分解菌群中分离到革兰氏阳性细胞壁的新嗜碱厌氧发酵菌Z-7981和Z-7981′。细胞是活动杆，虽然尺寸不同，但根据98%的DNA/DNA同源性，它们属于同一物种。选择菌株Z-7981作为类型进行了详细的研究。它不产生孢子，其细胞不耐热。它是一种真正的亲碱剂，生长范围为pH 7.1 ~ pH 10.1，最适生长pH为pH 9.1，对Na+和碳酸盐离子有专性依赖，对Cl-不依赖。在总钠含量为0.076 ~ 1.27 M Na+的培养基中生长，在0.25 ~ 0.86 M Na+的培养基中生长最佳。生长在35°c时表现最佳，46°c以上没有生长。该菌具有耐气性，能够在不还原性培养基中以低于4.75%的O-2气相发酵。菌株Z-7981发酵单糖和双糖、糖醇，但氨基酸中只有谷氨酸和半胱氨酸，以及蛋白质底物、几丁质和干螺旋藻生物量。发酵产物为乙酸和乙醇。Fe3+在不产生能量的过程中被还原。在系统发育上，该新生物属于G+C含量较低的革兰氏阳性菌的第XI簇，与它最接近的分类群是Tindallia magadiensis。然而，根据其表型和基因型特征，它不属于任何已知的属。本文提出了一新属（新种），命名为Anoxynatronum sibiricum，菌株Z-7981为其类型（=DSM15060）。
New alkaliphilic anaerobic fermentative bacteria, strains Z-7981 and Z-7981', with Gram-positive cell walls, were isolated from the cellulolytic community from the soda lake Nizhnee Beloye, south-east of Baikal. Cells were motile rods, which differed in dimensions but, according to 98% DNA/DNA homology, belonged to the same species. Strain Z-7981 was chosen as the type and studied in detail. It did not produce spores and its cells were non-thermoresistant. It was a true alkaliphile with a growth range from pH 7.1 to pH 10.1 and optimal pH for growth at pH 9.1 It was obligately dependent on Na+ and carbonate ions but not on Cl-. Growth occurred in media with total sodium content from 0.076 M to 1.27 M Na+ with a broad optimum from 0.25 to 0.86 M Na+. Growth showed an optimum at 35degreesC, with absence of growth above 46degreesC. The organism was aerotolerant and was capable of fermentation in non-reducing medium at less than 4.75% O-2 in the gas phase. Strain Z-7981 fermented mono- and disaccharides, sugar alcohols, but only glutamate and cysteine among the amino acids, and the proteinaceous substrates, chitin and dried Spirulina biomass. Fermentation products were acetate and ethanol. Fe3+ was reduced in a process that yielded no energy. Phylogenetically the new organism belonged to cluster XI of the Gram-positive bacteria with low G+C content and its closest neighboring taxon was Tindallia magadiensis. However, according to its phenotypic and genotypic characters it did not belong to any known genus from this group. We suggest a new genus and species with the name Anoxynatronum sibiricum and strain Z-7981 as its type (=DSM15060).