Determination of microcystin-LR in surface water by a magnetic bead-based colorimetric immunoassay using antibody-conjugated gold nanoparticles
Determination of microcystin-LR in surface water by a magnetic bead-based colorimetric immunoassay using antibody-conjugated gold nanoparticles
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DOI：
10.1039/c5ay02164b
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Knopp, D.
中科院分区：
文献类型：
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作者：
Neumann, A. -C.;Wang, X.;Knopp, D.
在此，我们描述了使用抗原功能化磁珠（MB）和抗体固定金纳米粒子（AuNP）结合已建立的金染色方法来开发均相竞争比色免疫测定法，用于测定地表水中的微囊藻毒素-亮氨酸-精氨酸（MC-LR）。固相萃取被证明是最有益的样品制备方法，可减少基质效应，基质效应可能导致 AuNP 聚集并导致测定失败。所开发的方法可以在0.51-11.68μgL-1的线性范围内检测MC-LR，检测限(LOD)为0.38μgL-1。加标水样中毒素的回收率为88.8%至117.5%。这种提出的免疫测定法构成了一种简单且经济有效的方法，用于检测淡水中 1 μg L-1 左右的 MC-LR 卫生相关浓度，无需任何昂贵或具有挑战性的设备。
Herein we describe the development of a homogeneous competitive colorimetric immunoassay using antigen-functionalized magnetic beads (MBs) and antibody-immobilized gold nanoparticles (AuNPs) combined with the established gold staining method for the determination of microcystin-leucine-arginine (MC-LR) in surface water. Solid phase extraction proved to be the most beneficial sample preparation method in order to reduce matrix effects which might lead to agglomeration of the AuNPs and failing of the assay. The developed method could detect MC-LR in a linear range from 0.51-11.68 mu g L-1 with a limit of detection (LOD) of 0.38 mu g L-1. The recovery rates of the toxin in spiked water samples were in the range from 88.8% to 117.5%. This proposed immunoassay constitutes a simple and cost-effective method for the detection of sanitary relevant concentrations around 1 mu g L-1 of MC-LR in fresh waters without any expensive or challenging equipment.