Adsorption and Self-Assembly of M13 Phage into Directionally Organized Structures on C and SiO2 Films
Adsorption and Self-Assembly of M13 Phage into Directionally Organized Structures on C and SiO2 Films
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DOI：
10.1021/la502534t
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发表时间：
2014-09-30
期刊：
影响因子：
3.9
通讯作者：
van Aken, Peter A.
中科院分区：
文献类型：
--
作者：
Moghimian, Pouya;Srot, Vesna;van Aken, Peter A.
展示了一种使用无定形碳和 SiO2 薄膜定向组装 M13 噬菌体的通用方法。在病毒纳米纤维倾向于无序的浓度范围内观察到M13噬菌体大分子对于掺入无定形碳表面上的对齐结构的高亲和力。相比之下，当病毒颗粒沉积在附近时，在 SiO2 薄膜上采用对齐方向的自由度较小。这里，根据表面化学和竞争性静电相互作用，提出了碳表面在显着增强吸附和生成具有高定向顺序的病毒阵列中的作用的解释。这项研究建议使用无定形碳基底作为定向组织紧密堆积的二维 M13 病毒膜的模板，这可能是矿化各种光滑且均匀的无机纳米结构层的有前途的途径。
A versatile method for the directional assembly of M13 phage using amorphous carbon and SiO2 thin films was demonstrated. A high affinity of the M13 phage macromolecules for incorporation into aligned structures on an amorphous carbon surface was observed at the concentration range, in which the viral nanofibers tend to disorder. In contrast, the viral particles showed less freedom to adopt an aligned orientation on SiO2 films when deposited in close vicinity. Here an interpretation of the role of the carbon surface in significant enhancement of adsorption and generation of viral arrays with a high orientational order was proposed in terms of surface chemistry and competitive electrostatic interactions. This study suggests the use of amorphous carbon substrates as a template for directional organization of a closely-packed and two-dimensional M13 viral film, which can be a promising route to mineralize a variety of smooth and homogeneous inorganic nanostructure layers.