Isolation of full-length cDNA and chromosomal localization of human NF-κB modulator NEMO to Xq28
Isolation of full-length cDNA and chromosomal localization of human NF-κB modulator NEMO to Xq28
复制标题
DOI：
10.1159/000025378
复制
发表时间：
1999-03-01
影响因子：
11
通讯作者：
Jeang, KT
中科院分区：
文献类型：
--
作者：
Jin, DY;Jeang, KT
NEMO是IKB激酶复合物的重要组成部分。其他人已经表明，小鼠NEMO的表达可以补充两种NEMO缺陷细胞系中对NF-κ B刺激的响应性的缺乏。在这里，我们报告的全长人NEMO cDNA的分离。人NEMO cDNA的虚拟翻译预测了一个48 kD的卷曲螺旋蛋白，与小鼠同源物具有87.9%的同一性和90.5%的相似性。通过序列比对，我们将人NEMO基因定位于染色体Xq 28。我们注意到NEMO和G6 PD（葡萄糖-6-磷酸脱氢酶）基因座以头对头方向排列，间隔不超过800 bp。该图谱位置进一步得到人NEMO mRNA的可变剪接变体序列的支持。因此，人NEMO是与G6 PD基因座紧密相邻的X连锁基因。
NEMO is an essential component of the IKB kinase complex. Others have shown that expression of mouse NEMO can complement the lack of responsiveness to NF-kappa B stimuli in two NEMO-deficient cell lines. Here we report the isolation of a full-length human NEMO cDNA. Virtual translation of human NEMO cDNA predicts a 48-kD coiled-coil protein which shares 87.9% identity and 90.5% similarity with the mouse homolog. By sequence alignment, we mapped the human NEMO gene to chromosome Xq28. We note that the NEMO and the G6PD (glucose-6-phosphate dehydrogenase) loci are arranged in a head-to-head orientation separated by no more than 800 bp. This map location is further supported by the sequence of an alternatively spliced variant of human NEMO mRNA. Thus, human NEMO is an X-linked gene closely adjacent to the G6PD locus.