Effect of monochromatic light on the temporal expression of N-acetyltransferase in chick pineal gland
Effect of monochromatic light on the temporal expression of N-acetyltransferase in chick pineal gland
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单色光对鸡松果体N-乙酰转移酶时间表达的影响
DOI：
10.1080/07420528.2020.1754846
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发表时间：
2020-04-20
影响因子：
2.8
通讯作者：
Chen Yaoxing
中科院分区：
文献类型：
--
作者：
Jiang Nan;Cao Jing;Chen Yaoxing
摘要鸟类松果体是一种独立的分子振荡器，它接受外界光信息，调节褪黑素的合成和分泌。芳基烷基胺N-乙酰基转移酶通过控制褪黑激素的节律性产生，在松果体中起着重要作用。先前的研究表明，单色光影响鸡松果体褪黑激素的分泌，褪黑素的分泌受分子生物钟基因的调控。本研究旨在研究单色光对雏鸡松果体cAanat、Clock蛋白(Clock和BMAL1)、cCreb和opsins(cOpnp，Pinopsin；cOpn4-1，Melopsin-1；cOpn4-2，Melosopsin-2)水平昼夜节律的影响。在白光(WL)、红光(RL)、绿光(GL)和蓝光(BL)、光(L)：暗(D)、12L：12D的光照周期下饲养2 40只0日龄肉鸡，结果表明，RL下cAanat、Clock、BMAL1、cCreb、cOpnp、cOpn4-1和cOpn4-2的表达有显著的昼夜节律，而cOpnp的表达没有明显的昼夜节律。与WL相比，GL使cAanat mRNA水平升高，而RL使其降低。同时，GL中高水平表达Clock和BMAL1蛋白。此外，GL的cOpnp基因表达高峰出现的时间早于WL、RL和BL。这些结果表明，单色光通过生物钟影响雏鸡松果体中cAanat的日常表达。GL激活cAanat的转录，RL抑制cAanat的转录。同时，GL可能会提前诱导cOpnp mRNA的峰值，从而影响光的传递。因此，单色光通过影响松果体Opsins的表达来影响时钟调节器的水平，从而调节小鸡松果体中的CAANAT。
ABSTRACT The avian pineal gland is an independent molecular oscillator that receives external light information that regulates the synthesis and secretion of melatonin. Arylalkylamine N-acetyltransferase plays an important role in the pineal gland by controlling the rhythmic production of melatonin. Previous study showed that monochromatic light influences the secretion of melatonin, which is regulated by the molecular circadian clock genes in chick pineal gland. This study was designed to investigate the effect of monochromatic light on the circadian rhythm of levels of cAanat, clock protein (CLOCK and BMAL1), cCreb, and opsins (cOpnp, Pinopsin; cOpn4-1, Melanopsin-1; cOpn4-2, Melanopsin-2) in chick pineal gland. A total of 240 post-hatching day (P) 0 broiler chickens were reared under white (WL), red (RL), green (GL), and blue light (BL) with light (L)-dark (D) cycle of 12L:12D for 14 d. The results show significant circadian rhythms in the expression of cAanat, CLOCK, BMAL1, cCreb, cOpnp, cOpn4-1, and cOpn4-2, but not for cOpnp under RL. Compared with WL, GL increased the level of cAanat mRNA, while RL decreased it. Meanwhile, CLOCK and BMAL1 proteins were expressed at high levels in GL. Furthermore, the peak of the 24 h pattern of cOpnp mRNA in GL was earlier than that of in WL, RL, and BL. These results demonstrated that monochromatic light affects the daily expression of cAanat in the chick pineal gland via the biological clock. GL activates the transcription of cAanat, while RL suppresses the transcription of cAanat. Meanwhile, GL appears to induce the peak of cOpnp mRNA in advance to affect the transmission of light. Thus, monochromatic light regulates cAanat in the chick pineal gland by affecting the levels of clock regulators via entraining the expression of pineal gland opsins.