Synergistic Inhibition of Enamel Demineralization by Peptide 8DSS and Fluoride
Synergistic Inhibition of Enamel Demineralization by Peptide 8DSS and Fluoride
复制标题
肽 8DSS 和氟化物协同抑制牙釉质脱矿
DOI：
10.1159/000442896
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发表时间：
2016-01-01
期刊：
影响因子：
4.2
通讯作者：
Zhang, Linglin
中科院分区：
文献类型：
--
作者：
Yang, Yang;Lv, Xueping;Zhang, Linglin
仿生肽8DSS在体外具有促进脱矿釉质再矿化的作用。在这里，我们检查了8DSS单独和与氟化物组合在模拟口内条件的pH循环期间抑制牙釉质脱矿的能力。在1，000 ppm NaF（阳性对照）、蒸馏去离子水（DDW;阴性对照）、单独25 μM 8DSS、25 μ M 8DSS与500 ppm NaF（8DSS-FL）或25 μ M 8DSS与1，000 ppm NaF（8DSS-FH）存在下，对釉质块进行9天pH循环，每天两次，每次1 min。块进行了分析，在表面显微硬度（SMH），氟吸收和矿物质含量。与DDW治疗组相比，8DSS治疗组的矿物质损失显著降低，病变较浅，SMH较高。在单独用8DSS或单独用氟化物处理的块之间没有观察到显著差异。单独用8DSS或DDW处理的块显示出相似的氟吸收量，这是所有处理组中最低的。用8DSS-FL或8DSS-FH处理的块没有显著差异，并且两组均显示出比NaF处理的块显著更大的SMH和氟化物摄取以及显著更低的矿物质损失和更浅的病变。从表面层（10 µm）到损伤深度（110 µ m），8DSS处理的块中的矿物质含量显著高于DDW处理的块，并且从10至90 µm，8DSS-FL或8DSS-FH处理的块中的矿物质含量显著高于NaF处理的块。这些发现说明了8DSS抑制釉质脱矿和增强NaF防龋效果的潜力。
The biomimetic peptide 8DSS has shown beneficial effects in promoting remineralization of demineralized enamel in vitro. Here we examined the ability of 8DSS alone and in combination with fluoride to inhibit enamel demineralization during pH-cycling mimicking intraoral conditions. Enamel blocks were subjected to 9 days of pH-cycling in the presence of 1,000 ppm NaF (positive control), distilled-deionized water (DDW; negative control), 25 μM 8DSS alone, 25 μM 8DSS with 500 ppm NaF (8DSS-FL) or 25 μM 8DSS with 1,000 ppm NaF (8DSS-FH) twice daily for 1 min each time. The blocks were analyzed in terms of surface microhardness (SMH), fluoride uptake and mineral content. The 8DSS-treated blocks showed significantly lower mineral loss, shallower lesions and higher SMH than the DDW-treated blocks. No significant differences were observed between the blocks treated with 8DSS alone or fluoride alone. The blocks treated with 8DSS alone or DDW showed similar amounts of fluoride uptake, which was the lowest of all the treatment groups. The blocks treated with 8DSS-FL or 8DSS-FH did not differ significantly, and both groups showed significantly greater SMH and fluoride uptake as well as significantly lower mineral loss and shallower lesions than the NaF-treated blocks. Mineral content was significantly higher in the 8DSS-treated blocks than in the DDW-treated blocks from the surface layer (10 µm) to the lesion depth (110 µm), and it was significantly higher in the blocks treated with 8DSS-FL or 8DSS-FH than in the NaF-treated blocks from 10 to 90 µm. These findings illustrate the potential of 8DSS for inhibiting enamel demineralization and for enhancing the anticaries effect of NaF.