Growth Media for Mixed Multispecies Oropharyngeal Biofilm Compositions on Silicone
Growth Media for Mixed Multispecies Oropharyngeal Biofilm Compositions on Silicone
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用于有机硅上混合多物种口咽生物膜组合物的生长培养基
DOI：
10.1155/2019/8051270
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发表时间：
2019
影响因子：
--
通讯作者：
B. Schneider
中科院分区：
文献类型：
--
作者：
M. Leonhard;Beata Zatorska;D. Moser;B. Schneider
目的：细菌和念珠菌在硅胶发音假体中的微生物定植限制了喉切除患者中现代发音假体的器械寿命。因此，研究的重点是新材料，涂料和表面增强的生物膜抑制性能。本体外研究的目的是评价7种常用的生长培养基，以模拟混合口咽菌种在假体硅胶上作为中尺度生物膜的生长，用于未来研究。方法和结果使用酵母蛋白胨葡萄糖培养基（YPD）、酵母氮基培养基（YNB）、M199培养基、Spider培养基、RPMI 1640培养基、胰蛋白酶大豆肉汤（TSB）和胎牛血清（FBS）在硅胶上培养组合的混合念珠菌菌株和混合细菌-真菌组合物超过22天。生物膜表面扩散和微观生长显示出体内生物膜的变化，这取决于微生物组成和生长培养基。结论YPD和FBS证明支持混合细菌-真菌口咽生物膜沉积物在数周内的连续体外生长，这是口咽生物膜组合物长期体外测试所需的。研究的意义和影响该研究提供了混合多物种生物膜组合物的培养条件的数据，可用于未来的假体设计。
Aims Microbial colonization of silicone voice prostheses by bacteria and Candida species limits the device lifetime of modern voice prostheses in laryngectomized patients. Thus, research focuses on biofilm inhibitive properties of novel materials, coatings, and surface enhancements. Goal of this in vitro study was the evaluation of seven commonly used growth media to simulate growth of mixed oropharyngeal species as mesoscale biofilms on prosthetic silicone for future research purposes. Methods and Results Yeast Peptone Dextrose medium (YPD), Yeast Nitrogen Base medium (YNB), M199 medium, Spider medium, RPMI 1640 medium, Tryptic Soy Broth (TSB), and Fetal Bovine Serum (FBS) were used to culture combined mixed Candida strains and mixed bacterial-fungal compositions on silicone over the period of 22 days. The biofilm surface spread and the microscopic growth showed variations from in vivo biofilms depending on the microbial composition and growth medium. Conclusion YPD and FBS prove to support continuous in vitro growth of mixed bacterial-fungal oropharyngeal biofilms deposits over weeks as needed for longterm in vitro testing with oropharyngeal biofilm compositions. Significance and Impact of Study The study provides data on culture conditions for mixed multispecies biofilm compositions that can be used for future prosthesis designs.
影响因子：
5.8
作者：
Nobbs AH;Jenkinson HF
通讯作者：
Jenkinson HF