NMDA induces apoptosis and necrosis in neuronal cultures. Increased APP immunoreactivity is linked to apoptotic cells
NMDA induces apoptosis and necrosis in neuronal cultures. Increased APP immunoreactivity is linked to apoptotic cells
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DOI：
10.1016/s0304-3940(96)13310-3
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发表时间：
1997-01-17
影响因子：
2.5
通讯作者：
Hugon, J
中科院分区：
文献类型：
--
作者：
Lesort, M;Esclaire, F;Hugon, J
在长期（16小时）暴露于低浓度N-甲基-D-天冬氨酸（NMDA；20μM）的大鼠神经元培养物中，观察到两种不同类型的神经元死亡：坏死和凋亡。坏死神经元的免疫细胞化学研究表明，细胞质区域不存在淀粉样前体蛋白（APP）标记。相反，神经元凋亡与细胞质 APP 免疫反应性的增加相关。此外，在一些凋亡神经元周围也检测到细胞外APP的存在，表明细胞凋亡可能导致细胞外APP的存在。 (C) 1997 爱思唯尔科学爱尔兰有限公司
In rat neuronal cultures exposed for a prolonged period (16 h) to a low concentration of N-methyl-D-aspartate (NMDA; 20 mu M) two different types of neuronal death were observed, necrosis and apoptosis. The immunocytochemical study of necrotic neurons revealed the absence of amyloid precursor protein (APP) labeling in the cytoplasmic region. In contrast, neuronal apoptosis was associated with an increase in cytoplasmic APP immunoreactivity. In addition, the presence of extracellular APP was also detected around some apoptotic neurons, suggesting that apoptosis could contribute to the presence of extracellular APP. (C) 1997 Elsevier Science Ireland Ltd.