Effects of linking 15-zinc finger domains on DNA binding specificity and multiple DNA binding modes
Effects of linking 15-zinc finger domains on DNA binding specificity and multiple DNA binding modes
复制标题
DOI：
10.1016/j.bmcl.2005.03.040
复制
发表时间：
2005-05-02
影响因子：
2.7
通讯作者：
Sugiura, Y
中科院分区：
文献类型：
--
作者：
Hirata, T;Nomura, W;Sugiura, Y
为了评估锌指结构域多连接的可能性，以了解DNA结合机制和基因调控，我们构建了最长的人造锌指蛋白Sp1ZF15。该锌指由5个单位Sp1锌指肽组成，由规范短连接序列（TGEKP）连接。确定了Sp1ZF15对50个碱基对的DNA识别和与更短靶标的潜在结合。GCG三联体到ATA在靶位点的序列改变清楚地表明Sp1ZF15根据靶序列改变其DNA结合模式。特别有趣的是，Sp1ZF15控制着与靶DNA的长度和序列相对应的DNA结合中活跃的指结构域的数量。这些结果表明，基于这些多锌指蛋白的人工转录因子在调节大量细胞过程方面具有很大的潜力。(c) 2005 Elsevier Ltd版权所有。
To assess the possibility of multi-connection of zinc finger domains for understanding of DNA binding mechanisms and gene regulation, the longest artificial zinc finger protein, Sp1ZF15, has been constructed. This zinc finger consists of 5 units of Sp1 zinc finger peptide connected by canonical short linker sequences (TGEKP). Recognition of the 50 base pairs of DNA and potential binding to shorter targets by Sp1ZF15 were determined. Sequence alterations of the GCG triplet to ATA at a target site clearly showed that Sp1ZF15 changes its DNA binding mode depending on the target sequences. Of special interest is the fact that Sp1ZF15 controls the number of finger domains active in DNA binding corresponding to the length and sequence of the target DNA. These results suggest that artificial transcription factors based upon these multi-zinc finger proteins have great potential for the regulation of a vast number of cellular processes. (c) 2005 Elsevier Ltd. All rights reserved.