Genetic control of storage oil synthesis in seeds of Arabidopsis
Genetic control of storage oil synthesis in seeds of Arabidopsis
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DOI：
10.1104/pp.104.049486
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发表时间：
2004-10-01
期刊：
影响因子：
7.4
通讯作者：
Hills, MJ
中科院分区：
文献类型：
--
作者：
Hobbs, DH;Flintham, JE;Hills, MJ
利用拟南芥生态型兰茨贝格直立和佛得角群岛杂交获得的重组近交群体，研究了控制种子含油量和脂肪酸组成的数量性状基因座（QTL）。多QTL模型定位确定了两个主效和两个次效QTL，占43%的油含量在人口中的变化。最显著的QTL位于第2染色体的底部，占遗传变异的17%。另外两个显著的QTL，位于1号染色体的上臂和下臂，占遗传变异的19%。靠近第3染色体顶端的QTL对第1染色体上臂的QTL具有上位性。与FAD 3位点共定位的亚油酸（18：2）和亚麻酸（18：3）含量的QTL较强，与FAD 2位点共定位的油酸（18：1）含量的QTL较强，棕榈酸（16：0）、硬脂酸（18：0）和二十碳烯酸（20：1）含量的QTL较弱。在染色体2上种子含油量QTL的存在通过产生在染色体2底部含有22-cM的兰茨贝格直立DNA区域的品系来证实，在基因组的其他区域中含有佛得角群岛，其在QTL分析中已显示影响含油量。
Quantitative trait loci (QTL) that control seed oil content and fatty acid composition were studied using a recombinant inbred population derived from a cross between the Arabidopsis ecotypes Landsberg erecta and Cape Verdi Islands. Multiple QTL model mapping identified two major and two minor QTL that account for 43% of the variation in oil content in the population. The most significant QTL is at the bottom of chromosome 2 and accounts for 17% of the genetic variation. Two other significant QTL, located on the upper and lower arms of chromosome 1, account for a further 19% of the genetic variation. A QTL near to the top of chomosome 3 is epistatic to that on the upper arm of chromosome 1. There are strong QTL for linoleic (18:2) and linolenic (18:3) acids contents that colocate with the FAD3 locus, another for oleic acid (18:1) that colocates with FAD2 and other less significant QTL for palmitic (16:0), stearic (18:0), and eicosaenoic (20:1) acids. The presence of the QTL for seed oil content on chromosome 2 was confirmed by the generation of lines that contain a 22-cM region of Landsberg erecta DNA at the bottom of chromosome 2 in a background containing Cape Verdi Islands in other regions of the genome that had been shown to influence oil content in the QTL analysis.