Construction of tissue-engineered corpus cavernosum with muscle-derived stem cells and transplantation in vivo
Construction of tissue-engineered corpus cavernosum with muscle-derived stem cells and transplantation in vivo
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DOI：
10.1111/j.1464-410x.2010.09695.x
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发表时间：
2011-05-01
影响因子：
4.5
通讯作者：
Zhang, Jinming
中科院分区：
文献类型：
--
作者：
Ji, Chenyang;Min, Fuli;Zhang, Jinming
关于这个问题有什么进展？这项研究增加了什么？阴茎海绵体的组织工程化研究已有报道，但尚未形成理想的方法。在这项研究中，肌肉来源的干细胞被用作种子细胞，以构建组织工程阴茎海绵体。结果表明，MDSCs可在脱细胞海绵体胶原基质三维支架上种植，并在体内发育成与天然阴茎海绵体相似的组织。目的探讨以肌源性干细胞（MDSCs）为种子细胞构建组织工程阴茎海绵体（TECC）的可行性，并测定其在体内的生长潜力。通过细胞去除程序从成年兔阴茎获得。使用消化方法和Preplate技术分离和纯化MDSC，然后以30 X IO 6个细胞/mL的浓度接种在ACCM上以构建TECC。培养5天后，将接种的ACCM植入兔的白蛋白。植入后2、4和6个月取出植入物。结果：脱细胞过程成功地提取了所有的细胞成分，同时保留了原胶原纤维。组织学分析显示，实验组在所有时间点的细胞数量和排列生长都比对照组多。对照组α-平滑肌肌动蛋白和内皮型一氧化氮合酶阳性细胞在实验group.CONCLUSIONOur研究表明，MDSCs可以种植在三维ACCM支架和发展组织，是类似于天然正常阴茎海绵体。
What's known on the subject? and What does the study add?The tissue-engineered research of corpus cavernosum has been studied, but an ideal method was not carried out. In the study, muscle-derived stem cells were used as seeding cells to construct tissue-engineered corpus cavernosums. The result demonstrated MDSCs could be seeded on three-dimensional scaffolds of acellular corporal collagen matrices and developed into tissues similar to native corpus cavernosum in vivo.OBJECTIVETo investigate the feasibility of tissue-engineered corpus cavernosum (TECC) with muscle-derived stem cells (MDSCs) as seed cells and determine the growth potential in vivo.MATERIALS AND METHODSAcellular corporal collagen matrices (ACCMs) were obtained from adult rabbit penis by a cell removal procedure. MDSCs were separated and purified using a digestion method and Preplate technique, then seeded on ACCMs at a concentration of 30 x 106 cells/mL to construct TECCs. After 5 days of culture, seeded ACCMs were implanted with albuginea of rabbits. The implants were retrieved at 2, 4 and 6 months after implantation.Histochemistry, immunohistochemisry and scanning electron microscopy were performed to analyse the morphological characteristics of the TECCs.RESULTSThe decellularization process successfully extracted all cellular components while preserving the original collagen fibres.Histological analyses of the explants at all time points in the experimental group had more cells and better arranged growth than the control group. alpha-Smooth muscle actin and endothelial nitric oxide synthase-positive cells were more prevalent in the experimental group.CONCLUSIONOur study showed that MDSCs can be seeded on three-dimensional ACCM scaffolds and develop tissues that are similar to native normal corpus cavernosum.